Anti-CROP antibody (ab231982)
Key features and details
- Rabbit polyclonal to CROP
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-CROP antibody
See all CROP primary antibodies -
Description
Rabbit polyclonal to CROP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Pig
Predicted to work with: Cow, Orangutan -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human CROP aa 1-250. Expressed in E.coli.
Database link: O95232 -
Positive control
- WB: Recombinant human CROP; mouse and pig cerebrum tissue lysate; HeLa whole cell lysate. IHC-P: Human liver and kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 51 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 51 kDa. |
Target
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Function
Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing. -
Tissue specificity
Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung. -
Sequence similarities
Belongs to the Luc7 family. -
Post-translational
modificationsPhosphorylated in vitro by SRPK1, SRPK2 and CLK1. -
Cellular localization
Nucleus speckle. The subnuclear localization is affected by cisplatin. - Information by UniProt
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Database links
- Entrez Gene: 539955 Cow
- Entrez Gene: 51747 Human
- Entrez Gene: 67684 Mouse
- Entrez Gene: 100172991 Orangutan
- Omim: 609434 Human
- SwissProt: Q3SX41 Cow
- SwissProt: O95232 Human
- SwissProt: Q5SUF2 Mouse
see all -
Alternative names
- cAMP regulatory element-associated protein 1 antibody
- Cisplatin resistance-associated overexpressed protein antibody
- Cisplatin resistance-associated-overexpressed protein antibody
see all
Images
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Formalin-fixed, paraffin-embedded human liver tissue stained for CROP using ab231982 at 20 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for CROP using ab231982 at 20 μg/ml in immunohistochemical analysis.
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Anti-CROP antibody (ab231982) at 2 µg/ml + Recombinant human CROP protein
Developed using the ECL technique.
Predicted band size: 51 kDa -
Anti-CROP antibody (ab231982) at 2 µg/ml + Mouse cerebrum tissue lysate
Developed using the ECL technique.
Predicted band size: 51 kDa -
Anti-CROP antibody (ab231982) at 2 µg/ml + Pig cerebrum tissue lysate
Developed using the ECL technique.
Predicted band size: 51 kDa -
Anti-CROP antibody (ab231982) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Developed using the ECL technique.
Predicted band size: 51 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231982 has not yet been referenced specifically in any publications.