Anti-CROP antibody (ab80412)
Key features and details
- Rabbit polyclonal to CROP
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CROP antibody
See all CROP primary antibodies -
Description
Rabbit polyclonal to CROP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between residue 1 and 50 of human CROP (NP_057508.2).
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Positive control
- IHC-P: Human testicular seminoma, mouse colon carcinoma tissue. WB: HeLa cells whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing. -
Tissue specificity
Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung. -
Sequence similarities
Belongs to the Luc7 family. -
Post-translational
modificationsPhosphorylated in vitro by SRPK1, SRPK2 and CLK1. -
Cellular localization
Nucleus speckle. The subnuclear localization is affected by cisplatin. - Information by UniProt
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Database links
- Entrez Gene: 539955 Cow
- Entrez Gene: 51747 Human
- Entrez Gene: 67684 Mouse
- Entrez Gene: 100172991 Orangutan
- Entrez Gene: 360602 Rat
- Omim: 609434 Human
- SwissProt: Q3SX41 Cow
- SwissProt: O95232 Human
see all -
Alternative names
- cAMP regulatory element-associated protein 1 antibody
- Cisplatin resistance-associated overexpressed protein antibody
- Cisplatin resistance-associated-overexpressed protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse colon carcinoma (right) tissue labelling CROP with ab80412 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of Human CROP in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80412 at 3 µg/mg lysate for IP (lane 1). ab80412 was used at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab80412 has not yet been referenced specifically in any publications.