![Immunocytochemistry/ Immunofluorescence - Anti-CRYZ antibody [2A9DD1 ] (ab110310) image](#CRYZ-Primary-antibodies-ab110310-7.gif)
![Immunoprecipitation - Anti-CRYZ antibody [2A9DD1 ] (ab110310) image](#CRYZ-Primary-antibodies-ab110310-8.gif)
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Product name
Anti-CRYZ antibody [2A9DD1 ]
See all CRYZ products (3) ...
Description
Mouse monoclonal [2A9DD1 ] to CRYZ
Tested applications
ICC/IF, IP, In-Cell ELISAmore details
Cross reactivity
Reacts with
Human
Positive control
HeLa cells; Human liver and HepG2 lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
ab110310 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
2A9DD1
Isotype
IgG2a
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Drug metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Nucleotide metabolism
Signal Transduction >> Metabolism >> Drug metabolism
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Neuroscience >> Sensory System >> Visual system
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 2 µg/ml. For 2 hours.
IP: Use at an assay dependent dilution.
In-Cell ELISA: Use at an assay dependent concentration.
Target
Function
Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.
Tissue specificity
Only very low amounts in the lens.
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ08257
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NADPH:quinone reductase antibody
- Zeta crystallin antibody
- Crystallin zeta (quinone reductase) antibody
see all
Database links
- Entrez Gene: 1429 Human
- Omim: 123691 Human
- SwissProt: Q08257 Human
- Unigene: 83114 Human
Anti-CRYZ antibody [2A9DD1 ] images:
Immunocytochemistry/ Immunofluorescence - Anti-CRYZ antibody [2A9DD1 ] (ab110310)
![Immunocytochemistry/ Immunofluorescence - Anti-CRYZ antibody [2A9DD1 ] (ab110310)](/ps/datasheet/images/110/ab110310/CRYZ-Primary-antibodies-ab110310-7.gif)
Immunocytochemistry analysis using ab110310 at 2µg/ml staining CRYZ in HELa cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green).
Immunoprecipitation - Anti-CRYZ antibody [2A9DD1 ] (ab110310)
![Immunoprecipitation - Anti-CRYZ antibody [2A9DD1 ] (ab110310)](/ps/datasheet/images/110/ab110310/CRYZ-Primary-antibodies-ab110310-8.gif)
Using MitoSciences' standard immunoprecipitation protocol, ab110310 precipitates the 35kDa CRYZ protein from human liver and HepG2 lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with silver nitrate.
References for Anti-CRYZ antibody [2A9DD1 ] (ab110310)
ab110310 has not yet been referenced specifically in any publications.
Publishing research using ab110310? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"