Recombinant Anti-CSHL1/CSL antibody [EPR12580] (ab174295)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12580] to CSHL1/CSL
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CSHL1/CSL antibody [EPR12580]
See all CSHL1/CSL primary antibodies -
Description
Rabbit monoclonal [EPR12580] to CSHL1/CSL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CSHL1/CSL. The exact sequence is proprietary.
Database link: Q14406 -
Positive control
- Human placenta tissue and tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12580 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Predicted molecular weight: 25 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 25 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be a novel gestational hormone required to compensate for absence of other members of the GH/CS cluster during gestation. -
Sequence similarities
Belongs to the somatotropin/prolactin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1444 Human
- Omim: 603515 Human
- SwissProt: Q14406 Human
- Unigene: 655225 Human
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Alternative names
- Chorionic somatomammotropin CS 5 antibody
- Chorionic somatomammotropin CS5 antibody
- Chorionic somatomammotropin hormone like 1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CSHL1/CSL with ab174295 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-CSHL1/CSL antibody [EPR12580] (ab174295) at 1/10000 dilution + Human placenta lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab174295 has been referenced in 1 publication.
- Wu D et al. How Placenta Promotes the Successful Reproduction in High-Altitude Populations: A Transcriptome Comparison between Adaptation and Acclimatization. Mol Biol Evol 39:N/A (2022). PubMed: 35642306