 image](#CSK-Primary-antibodies-ab14996-1.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Overview
Product name
Anti-CSK antibody [CSK-04]
See all CSK products (9) ...
Description
Mouse monoclonal [CSK-04] to CSK
Tested applications
IP, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant fragment: VSDFGLTKEAS STQDTGKLPV KWTAPEALRE KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYEVMK NCWHLDAAMR PSFLQLREQL EHIKTHELHL, corresponding to amino acids 330-450 of Human CSK.
VSDFGLTKEA S STQDTGKLPV KWTAPEALRE KKFSTK SDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KG YKMDAPDG CPPAVYEVMK NCWHLDAAMR PSFLQLREQ L EHIKTHELHL
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Purified from ascities.
Clonality
Monoclonal
Clone number
CSK-04
Isotype
IgG1
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CSK antibody [CSK-04](ab14996)
 image](/ps/datasheet/images/14/ab14996/CSK-Primary-antibodies-ab14996-1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
WB
- 1 Image
IHC-P
Application notes
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution. The immunosorbent (ab14996 covalently bound to beads) immunoprecipitates CSK from cell lysates.
WB: 1/250 - 1/300. Predicted molecular weight: 54 kDa. Weak non-specific band of approx. 60 kDa is detected in human cell lines in lower antibody dilutions.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
Tissue specificity
Expressed in lung and macrophages.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Post-translational
modifications
Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
Cellular localization
Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
Target information above from: UniProt accessionP41240
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Tyrosine protein kinase CSK antibody
- zgc:154049 antibody
- C SRC antibody
see all
Database links
- Entrez Gene: 1445 Human
- Entrez Gene: 12988 Mouse
- Omim: 124095 Human
- SwissProt: P41240 Human
- SwissProt: P41241 Mouse
- SwissProt: Q3UVH2 Mouse
- SwissProt: Q8VCW1 Mouse
- Unigene: 77793 Human
see all
Anti-CSK antibody [CSK-04] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CSK antibody [CSK-04](ab14996)
Ab14996 staining human normal skin tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-CSK antibody [CSK-04] (ab14996)
ab14996 has not yet been referenced specifically in any publications.
Publishing research using ab14996? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"