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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CSK antibody [CSK-04](ab14996)
](\ps\datasheet\images\14\ab14996\CSK-Primary-antibodies-ab14996-1.jpg)
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Product Name
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CSK antibody [CSK-04]
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See all CSK antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [CSK-04] to CSK
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Immunogen
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Recombinant fragment: VSDFGLTKEAS STQDTGKLPV KWTAPEALRE KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYEVMK NCWHLDAAMR PSFLQLREQL EHIKTHELHL, corresponding to amino acids 330-450 of Human CSK.
BLAST 'VSDFGLTKEA S STQDTGKL PV KWTAPEA LRE KKFSTK SDVW SFGIL LWEIY SFGR VPYPRI PLK DVVPRVE KG YKMDAPDG C PPAVYEVMK NCWHLDAAMR PSFLQLREQ L EHIKTHEL ' with  or 
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Reacts with
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Hu, Ms
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Tested applications
(see key)
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IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution. The immunosorbent (ab14996 covalently bound to beads) immunoprecipitates CSK from cell lysates.
WB: 1/250 - 1/300. Predicted molecular weight: 54 kDa. Weak non-specific band of approx. 60 kDa is detected in human cell lines in lower antibody dilutions.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
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Relevance
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C terminal Src kinase (CSK) is a non receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C terminal tyrosine of Src family kinases thereby down regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft associated protein PAG (CSK binding protein).
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CSK antibody [CSK-04] (ab14996) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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CSK-04
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from ascities.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CSK antibody [CSK-04] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CSK antibody [CSK-04] images, references, products related to ab14996 and other tools.
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CSK antibody [CSK-04] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CSK antibody [CSK-04](ab14996)
Ab14996 staining human normal skin tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to CSK
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CSK antibody [CSK-04] - more information
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Customer reviews (feedback) regarding CSK antibody [CSK-04] |
Customer FAQs regarding CSK antibody [CSK-04] |
Protocols for CSK antibody [CSK-04] |
Price and availability of products related to CSK antibody [CSK-04] |
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CSK antibody [CSK-04] - related products:
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CSK antibody [CSK-04] - other tools:
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Contact Abcam with a Technical Enquiry about CSK antibody [CSK-04] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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