Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Overview
Product name
Anti-CSK antibody
See all CSK products (9) ...
Description
Mouse monoclonal to CSK
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE , corresponding to amino acids 1-101 of Human CSK
MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 1 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
Tissue specificity
Expressed in lung and macrophages.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Post-translational
modifications
Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
Cellular localization
Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
Target information above from: UniProt accessionP41240
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Tyrosine protein kinase CSK antibody
- zgc:154049 antibody
- C SRC antibody
see all
Database links
- Entrez Gene: 1445 Human
- Omim: 124095 Human
- SwissProt: P41240 Human
- Unigene: 77793 Human
Anti-CSK antibody images:
IHC-P - CSK antibody (ab54684)
CSK antibody (ab54684) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.
Western blot - CSK antibody (ab54684)
CSK antibody (ab54684) at 1ug/lane + HL-60 cell lysate at 25ug/lane.
References for Anti-CSK antibody (ab54684)
ab54684 has not yet been referenced specifically in any publications.
Publishing research using ab54684? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"