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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Overview
Product name
Anti-CSK antibody
See all CSK products (9) ...
Description
Goat polyclonal to CSK
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Dog
Immunogen
Synthetic peptide: EQLEHIKTHELHL, corresponding to C terminal amino acids 438-450 of Human CSK.
C-EQLEHIKT HELHL
Positive control
Jurkat cell lysate, Human colon tissue.
General notes
GenBank Accession Number – NP_004374.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
IHC-P: Use a concentration of 3 - 6 µg/ml.
Target
Function
Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
Tissue specificity
Expressed in lung and macrophages.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Post-translational
modifications
Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
Cellular localization
Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
Target information above from: UniProt accessionP41240
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Tyrosine protein kinase CSK antibody
- zgc:154049 antibody
- C SRC antibody
see all
Database links
- Entrez Gene: 1445 Human
- Entrez Gene: 12988 Mouse
- Entrez Gene: 315707 Rat
- Omim: 124095 Human
- SwissProt: Q0VBZ0 Cow
- SwissProt: P41240 Human
- SwissProt: P41241 Mouse
- SwissProt: Q3UVH2 Mouse
see all
Anti-CSK antibody images:
Western blot - CSK antibody (ab744)
Predicted band size : 52 kDa
ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CSK antibody (ab744)
ab744 at 4µg/ml staining CSK in Human Colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Western blot - CSK antibody (ab744)
Anti-CSK antibody (ab744) at 1 µg/ml + Mouse spleen lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 52 kDa
References for Anti-CSK antibody (ab744)
This product has been referenced in:
- Ribeiro-Oliveira Aet al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB; Human.Read more (PubMed: 18701577) »
See 1 publication for this product
Publishing research using ab744? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"