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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg +2 and Ca +2), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Affinity purified from rabbit antiserum by affinity chromatography using
epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 51 kDa.
Function Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
Tissue specificity Expressed in lung and macrophages.
Sequence similarities Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
Post-translational modifications Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
Cellular localization Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
Information by UniProt
C SRC antibody
C SRC kinase antibody
C src Tyrosine Kinase antibody
Anti-CSK (phospho S364) antibody images
Western blot - CSK (phospho S364) antibody (ab61782)
All lanes : Anti-CSK (phospho S364) antibody (ab61782) at 1/500 dilution Lane 1 : extracts from HeLa cells, treated
with PMA (125ng/ml, 30mins) with no immunizing peptide Lane 2 : extracts from HeLa cells, treated
with PMA (125ng/ml, 30mins) with immunizing peptide Predicted band size : 51 kDa Observed band size : 55 kDa ( why is the actual band size different from the predicted?)
References for Anti-CSK (phospho S364) antibody (ab61782)
This product has been referenced in:
et al. Receptor desensitization and blockade of the suppressive effects of prostaglandin E(2) and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes. Cancer Immunol Immunother 60:111-22 (2011).
Read more (PubMed: 20960188) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"