Overview

  • Product nameAnti-CSK (phospho S364) antibody
    See all CSK primary antibodies
  • Description
    Rabbit polyclonal to CSK (phospho S364)
  • SpecificityDetects endogenous levels of CSK only when phosphorylated at serine 364.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human CSK around the phosphorylation site of serine 364 (KFSPTK)

  • Positive control
    • Extracts from HeLa cells, treated with PMA

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab61782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 51 kDa.
ELISA 1/40000.

Target

  • FunctionSpecifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
  • Tissue specificityExpressed in lung and macrophages.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
  • Cellular localizationCytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • C SRC antibody
    • C SRC kinase antibody
    • C src Tyrosine Kinase antibody
    • C-SRC kinase antibody
    • c-src tyrosine kinase antibody
    • Csk A antibody
    • CSK antibody
    • CSK_HUMAN antibody
    • CYTOPLASMIC TYROSINE KINASE antibody
    • EC 2.7.10.2 antibody
    • MGC112926 antibody
    • MGC117393 antibody
    • MGC154049 antibody
    • P60 Src antibody
    • Protein tyrosine kinase CYL antibody
    • Protein-tyrosine kinase CYL antibody
    • Proto oncogene tyrosine protein kinase antibody
    • Tyrosine protein kinase CSK antibody
    • Tyrosine-protein kinase CSK antibody
    • zgc:154049 antibody
    see all

Anti-CSK (phospho S364) antibody images

  • All lanes : Anti-CSK (phospho S364) antibody (ab61782) at 1/500 dilution

    Lane 1 : extracts from HeLa cells, treated with PMA (125ng/ml, 30mins) with no immunizing peptide
    Lane 2 : extracts from HeLa cells, treated with PMA (125ng/ml, 30mins) with immunizing peptide


    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-CSK (phospho S364) antibody (ab61782)

This product has been referenced in:
  • Su Y  et al. Receptor desensitization and blockade of the suppressive effects of prostaglandin E(2) and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes. Cancer Immunol Immunother 60:111-22 (2011). WB ; Human . Read more (PubMed: 20960188) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab61782.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"