The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/500.
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
ab74407 is likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CSTF3 (cleavage stimulation factor subunit 3) is required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs and is part of the CSTF complex. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 functions as a homodimer and interacts directly with CSTF1 and CSTF2.