Anti-CTBP2 antibody - C-terminal (ab190933)
Key features and details
- Rabbit polyclonal to CTBP2 - C-terminal
- Suitable for: IHC-Fr, WB, IHC-P, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CTBP2 antibody - C-terminal
See all CTBP2 primary antibodies -
Description
Rabbit polyclonal to CTBP2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide corresponding to Human CTBP2 aa 400 to the C-terminus (C terminal).
Database link: P56545 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 49 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 0.5 - 1 µg/ml.
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Notes |
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IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 49 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use a concentration of 0.5 - 1 µg/ml. |
Target
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Function
Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses. -
Tissue specificity
Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas. -
Sequence similarities
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. -
Post-translational
modificationsIsoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation. -
Cellular localization
Nucleus. Cell junction > synapse. - Information by UniProt
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Database links
- Entrez Gene: 281730 Cow
- Entrez Gene: 1488 Human
- Entrez Gene: 13017 Mouse
- Entrez Gene: 81717 Rat
- Omim: 602619 Human
- SwissProt: Q0VCQ1 Cow
- SwissProt: P56545 Human
- SwissProt: P56546 Mouse
see all -
Alternative names
- C terminal binding protein 2 antibody
- C-terminal-binding protein 2 antibody
- CtBP2 antibody
see all
Images
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All lanes : Anti-CTBP2 antibody - C-terminal (ab190933) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lane 5 : COLO320 cell lysate
Lane 6 : U87 cell lysate
Lane 7 : SW620 cell lysate
Predicted band size: 49 kDa -
CTBP2 was detected in frozen section of mouse intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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CTBP2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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CTBP2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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CTBP2 was detected in immunocytochemical section of human Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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CTBP2 was detected in frozen section of rat intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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CTBP2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab190933 has not yet been referenced specifically in any publications.