Recombinant Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7611(B)] to CTBP2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CTBP2 antibody [EPR7611(B)]
See all CTBP2 primary antibodies -
Description
Rabbit monoclonal [EPR7611(B)] to CTBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and SK-BR-3 cell lysates. Mouse brain and Rat brain tissue lysates. Flow Cyt (Intra): MCF7 cells. ICC/IF: SH-SY5Y cells IHC-P: Rat stomach, Mouse stomach, Human ovarian carcinoma, and Human glioma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7611(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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IHC-P |
1/6000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For mouse and rat, use at 1:12000. For unpurified, use at 1/250 - 1/500. |
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ICC/IF |
1/50.
For unpurified use at 1/100 - 1/250. |
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Flow Cyt (Intra) |
1/20.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
IHC-P
1/6000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For mouse and rat, use at 1:12000. For unpurified, use at 1/250 - 1/500. |
ICC/IF
1/50. For unpurified use at 1/100 - 1/250. |
Flow Cyt (Intra)
1/20. |
Target
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Function
Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses. -
Tissue specificity
Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas. -
Sequence similarities
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. -
Post-translational
modificationsIsoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation. -
Cellular localization
Nucleus. Cell junction > synapse. - Information by UniProt
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Database links
- Entrez Gene: 1488 Human
- Entrez Gene: 13017 Mouse
- Entrez Gene: 81717 Rat
- Omim: 602619 Human
- SwissProt: P56545 Human
- SwissProt: P56546 Mouse
- SwissProt: Q9EQH5 Rat
- Unigene: 501345 Human
see all -
Alternative names
- C terminal binding protein 2 antibody
- C-terminal-binding protein 2 antibody
- CtBP2 antibody
see all
Images
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Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling CTBP2 with purified ab128871 at 1:50 dilution (2.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling CTBP2 with purified ab128871 at 1:12000 (0.011 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling CTBP2 with purified ab128871 at 1:6000 (0.021 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling CTBP2 with purified ab128871 at 1/20 dilution (5 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling CTBP2 with Purified ab128871 at 1:12000 (0.011 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling CTBP2 with purified ab128871 at 1:6000 (0.021 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab128871 has been referenced in 10 publications.
- Zhang W et al. Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal Cancer. Front Oncol 12:805290 (2022). PubMed: 35299743
- Song F et al. Hidden hearing loss is associated with loss of ribbon synapses of cochlea inner hair cells. Biosci Rep 41:N/A (2021). PubMed: 33734328
- Parfenyev SE et al. Proteomic Analysis of Zeb1 Interactome in Breast Carcinoma Cells. Molecules 26:N/A (2021). PubMed: 34074001
- Zhou P et al. Transforming growth factor beta (TGF-ß) is activated by the CtBP2-p300-AP1 transcriptional complex in chronic renal failure. Int J Biol Sci 16:204-215 (2020). PubMed: 31929749
- Li S et al. FGF22 promotes generation of ribbon synapses through downregulating MEF2D. Aging (Albany NY) 12:6456-6466 (2020). PubMed: 32271716
- Wang H et al. Decreased levels of superoxide dismutase in inner pillar cells contribute to ribbon synapse impairment in presbycusis. Am J Transl Res 11:2403-2412 (2019). PubMed: 31105846
- Crespo-Garcia S et al. Individual and temporal variability of the retina after chronic bilateral common carotid artery occlusion (BCCAO). PLoS One 13:e0193961 (2018). PubMed: 29547662
- Dai F et al. CtBP2 overexpression promotes tumor cell proliferation and invasion in gastric cancer and is associated with poor prognosis. Oncotarget 8:28736-28749 (2017). IHC . PubMed: 28404932
- Wang Y et al. Expression and prognostic significance of CTBP2 in human gliomas. Oncol Lett 12:2429-2434 (2016). WB, IHC-P ; Human . PubMed: 27698809
- Karaca E et al. Oxygen-dependent acetylation and dimerization of the corepressor CtBP2 in neural stem cells. Exp Cell Res 332:128-35 (2015). PubMed: 25447313