Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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ab115042 |
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ab70303 has been referenced in 7 publications.
Publishing research using ab70303? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-CTCF antibody - ChIP Grade (ab70303) at 0.03 µg/ml
Lane 1 : MCF7 whole cell lysate at 50 µg
Lane 2 : MCF7 whole cell lysate at 15 µg
Lane 3 : MCF7 whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Lane 6 : HeLa whole cell lysate at 50 µg
Predicted band size : 83 kDa
Observed band size : 83 kDa
Exposure time : 30 seconds
Detection of Human CTCF by Western Blot of Immunprecipitate. Lane 1: MCF7 whole cell lysates immunoprecipitated using ab70302 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 2: MCF7 whole cell lysates immunoprecipitated using ab70303 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 3: MCF7 whole cell lysates immunoprecipitated using ab70304 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 4: control IgG was used for immunoprecipitation. All lanes: ab70303 at 0.03µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds.
Analysis of Human CTCF by Chromatin Immunoprecipitation (ChIP). Primary human fibroblasts from lung (5 x 107 cells) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 20µg ab70303. The resulting ChIP DNA was quantified using real-time PCR with primers against the IGF2-H19 ICR or a control region in the HoxA cluster.
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