Anti-CTCF antibody - ChIP Grade (ab70303)

I'm doing western blotting with mouse brain extract of hypothalamic tissue; no bands are observed; primary used at 1:250-1:1000 and secondary at 1:1000; protein sample up to 60ug; other antibodies work well with same samples

ANSWER:

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedtwo free of charge replacements:

One vial of ab18554 is being replaced with one vial of ab9574.

One vial of ab70303 is being replaced with one vial of ab126765.


To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Hello, dear AbCam representative! I simply wanted to confirm whether the antigen for this antibody is phosphorylation-specific or not? (I.e., when performing ChIP, should we use phosphatases, or does the antibody recognize an unphosphorylated region)? Thank you! (regarding Anti-CTCF Ab ab70303)

ANSWER:

Thank you for contacting Abcam regarding ab70303.


Regarding the immunogen used to generate this antibody, it is not specifically phosphorylated and thus this antibody does not specifically target phosphorylated CTCF. Thus phosphatase inhibitors are not required.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Kindly advise the difference between the immunogens of ab37478 and ab70303.
Thanks in advance for your assistance and reply.
Have a nice week,

ANSWER:

Thank you for contacting us.
The immunogen sequence of ab37478 is from N-terminal end between aa1-278 and the immunogen for ab70303 is from C-terminal end amino acid 650 - 700.
ab37477 immunogen is also derived from N-terminal end which could be a good alternative to ab37478.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.