Anti-CTGF antibody (ab5097)

Western blot

All lanes : Anti-CTGF antibody (ab5097) at 1/500 dilution

Lane 1 : recombinant human CTGF at 0.01 µg
Lane 2 : recombinant human CTGF at 0.01 µg with CTGF peptide (ab13741) at 1 µg/ml
Lane 3 : recombinant human CTGF at 0.1 µg
Lane 4 : recombinant human CTGF at 0.1 µg with CTGF peptide (ab13741) at 1 µg/ml

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution

Performed under reducing conditions.

Predicted band size : 38 kDa
Observed band size : 38 kDa


Exposure time : 10 seconds

Expected molecular weight: 38kDa
Not yet tested against endogenous CTGF

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CTGF antibody (ab5097)

ab5097 staining CTGF in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in buffer and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/50 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTGF antibody (ab5097)

ab5097 staining CTGF in human bronchus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed in formaldehyde and an antigen retrieval step was performed using EDTA pH9.0. Samples were then incubated with ab5097 at a 1/400 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit polyclonal.

This image is courtesy of an anonymous Abreview.

Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody (ab5097)

ICC/IF image of ab5097 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5097 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.