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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Overview
Product name
Anti-CTGF antibody
See all CTGF products (7) ...
Description
Rabbit polyclonal to CTGF
Specificity
This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741).
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CTGF.
(Peptide available as ab13741.)
Positive control
recombinant human CTGF;
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Secreted Molecules >> CCN Family
Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. (PubMed: 17028989)
WB: 1/500Predicted molecular weight: 38 kDa.Can be blocked with CTGF peptide (ab13741).
Target
Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Tissue specificity
Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted.
Target information above from: UniProt accessionP29279
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
see all
Database links
- Entrez Gene: 1490 Human
- Entrez Gene: 396809 Pig
- Entrez Gene: 64032 Rat
- Omim: 121009 Human
- SwissProt: P29279 Human
- SwissProt: Q5M8T4 Human
- SwissProt: Q6FHL8 Human
- SwissProt: O19113 Pig
see all
Anti-CTGF antibody images:
Western blot
All lanes : Anti-CTGF antibody (ab5097) at 1/500 dilution
Lane 1 : recombinant human CTGF at 0.01 µg
Lane 2 : recombinant human CTGF at 0.01 µg with CTGF peptide (ab13741) at 1 µg/ml
Lane 3 : recombinant human CTGF at 0.1 µg
Lane 4 : recombinant human CTGF at 0.1 µg with CTGF peptide (ab13741) at 1 µg/ml
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 38 kDa
Observed band size : 38 kDa
Exposure time : 10 seconds
Expected molecular weight: 38kDa
Not yet tested against endogenous CTGF
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CTGF antibody (ab5097)
ab5097 staining CTGF in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in buffer and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/50 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTGF antibody (ab5097)
ab5097 staining CTGF in human bronchus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and an antigen retrieval step was performed using EDTA pH9.0. Samples were then incubated with ab5097 at a 1/400 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit polyclonal.
This image is courtesy of an anonymous Abreview.
References for Anti-CTGF antibody (ab5097)
This product has been referenced in:
- van de Laar IMet al. Mutations in SMAD3 cause a syndromic form of aortic aneurysms and dissections with early-onset osteoarthritis. Nat Genet 43:121-6 (2011). IHC-P; Human.Read more (PubMed: 21217753) »
- Hoerder-Suabedissen Aet al. Novel markers reveal subpopulations of subplate neurons in the murine cerebral cortex. Cereb Cortex 19:1738-50 (2009).Read more (PubMed: 19008461) »
See all 4 publications for this product
Publishing research using ab5097? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"