Anti-CTGF antibody (ab51704)

I appreciate you taking the time to do this and will try one of the antibodies (ab51704.

ANSWER:

Thank you for your email. I am sending you the testing discount code for trying ab51704 in rabbit.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab51704 in rabbit. This code will give you: 1 freePRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for rabbitand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: http://www.abcam.com/collaborationdiscount.

looking for Ab to work in IHC-P and IHC-Fr and rabbit
check sequence homology for Abs tested in IHC-P/-Fr

ANSWER:

Thank you for contacting us.

I have checked the sequence homology of the immunogen with the rabbit sequence (http://www.uniprot.org/uniprot/B7NZB9) for the 3 antibodies that have been tested in IHC-P and IHC-Fr:

ab6992: mouse - rabbit: 97% homology
ab51704: human - rabbit: 97% homology
ab59681: human - rabbit: 93% homology, but the C-terminal region aligns better than N-terminal region.

All 3 antibodies' immunogens aligns very well with the rabbit sequence and can therefore be tried with this species. You would be eligible to use our testing discount program in this case.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: http://www.abcam.com/collaborationdiscount.

Please let me know if I shall send you a testing discount code for trying one of these antibodies in rabbit.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your answer.

I am interested in using one of the first 2 antibodies (ab5097 and ab6992) as there is less informations on the datasheet for the other 2, and both show a band at 13kDa rather than 38kDa.

As ab5097 was made against human CTGF we would naturally go for that one, but

The literature search showed only two articles using ab5097 antibody, both tested the antibody on human tissue (Van Der Laar et al., tested the antibody on human endothelial cells from the aortic wall, and Bellahence et al., tested the antibody on breast cancer cell lines [MDA-MB-31, B02]). Are you aware of any other study that was done on haemopoietic cells?
Ref: Van Der Laar IMBH et al. Mutations in SMAD3 cause a syndromic form of aortic aneurysms and dissections with early-onset osteoarthritis. Nature Genetics 2011: 43;121-126.
Bellahcene etal. Transcriptome analysis reveals an osteoblast-like phenotype for human osteotropic breast cancer cells. Breast Cancer Research 2007: 101(2);135-148.

On the other hand, ab6992 antibody was used by many studies, and some of them showed good and clear results on western blot and IHC. However, most of them are on rat/mice and we couldn’t find any study that used this antibody on haemopoietic cells, are you aware of any?

We are planning to use one of them on the following applications:
1. Western Blotting.
2. Immunohistochemistry.
3. Fluorescent Activated Cell Sorting (FACS).

It would be great if you could advice me on which one would be the most appropriate for my study.

Finally, what is the price of both antibody?

Thank you for your time and support.

Regards,

ANSWER:

Thank you for your reply.

I can confirm the following information for these two antibodies:

ab5097 Anti-CTGF antibody
This antibody is tested in WB and IHC on paraffin embedded sections (IHC-P). All tested and guaranteed applications are listed on the product datasheets and this information is updated as soon as we receive new information. I am sorry this is not tested inin flow cytometry or IHC-Fr (frozen sections).

Any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in many cases, regrettably we do not have access to all the articles mentioning our products, or subscriptions to all the journals that publish them. I'm sorry there are no further publications we are aware of for ab5097.

ab6992 Anti-CTGF antibody
I can confirm thatall the information we have is on the datasheet and we regrettably have no data fromhaemopoietic cells for this antibody. The antibody is tested and guaranteed for WB, IHC-P, IHC-Fr, but not for flow cytometry.

Regarding the WB results shown on the datasheets forab51704 and ab59681 are from recombinant. or fusion protein samples, which is why the molecular weight is not as expected size for CTGF.

If you would like to test ab6992 or ab5097 in flow cytometry, please contact me again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

http://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.