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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Overview
Product name
Anti-CTGF antibody
See all CTGF products (7) ...
Description
Mouse polyclonal to CTGF
Tested applications
WB, ELISA, IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken, Cow, Pig, Xenopus laevis
Immunogen
Recombinant E. coli derived CTGF, corresponding to amino acids 182-250 of Human CTGF with a HIS tag fusion protein
AYRLEDTFGP DPTMIRANCL VQTTEWSACS KTCGMGISTR VTNDNASCRL EKQSRLCMVR PCEADLEENI
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Secreted Molecules >> CCN Family
Cardiovascular >> Angiogenesis >> Growth Factors >> Other
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Western blot - CTGF antibody (ab51704)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Predicted molecular weight: 38 kDa.
ELISA
ELISA: 1/2000ELISA: 1/2000
IHC-Fr
IHC-Fr: 1/100IHC-Fr: 1/100
IHC-P
IHC-P: 1/50IHC-P: 1/50
Target
Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Tissue specificity
Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted.
Target information above from: UniProt accessionP29279
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
see all
Database links
- Entrez Gene: 374135 Chicken
- Entrez Gene: 281103 Cow
- Entrez Gene: 1490 Human
- Entrez Gene: 14219 Mouse
- Entrez Gene: 396809 Pig
- Entrez Gene: 64032 Rat
- Entrez Gene: 398003 Xenopus laevis
- Omim: 121009 Human
see all
Anti-CTGF antibody images:
Western blot - CTGF antibody (ab51704)
Lane 1 : Pre Bleed serum
Lane 2 : Anti-CTGF antibody (ab51704) at 1/5000 dilution
Lane 1 : E. coli derived CTGF (aa.182-250) fusion protein
Lane 2 : E. coli derived CTGF (aa.182-250) fusion protein
Predicted band size : 38 kDa
Observed band size : 13 kDa (why is the actual band size different from the predicted?)
References for Anti-CTGF antibody (ab51704)
This product has been referenced in:
- Le Pape Eet al. Microarray analysis sheds light on the dedifferentiating role of agouti signal protein in murine melanocytes via the Mc1r. Proc Natl Acad Sci U S A 106:1802-1807 (2009). WB; Mouse.Read more (PubMed: 19174519) »
See 1 publication for this product
Publishing research using ab51704? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"