Specific protocols
| |
To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you get on. |
| |
General protocols
Useful resources:Western blotting (WB) protocols:Immunohistochemistry (IHC) / Immunocytochemistry (ICC) protocols:Chromatin Immunoprecipitation (ChIP) protocols:Dot blot protocols:ELISA protocols:ELISPOT protocols: Flow cytometry / FACS protocols:Immunoprecipitation (IP) protocols: |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Paraffin-embedded sections) - CTGF antibody (ab6992)
ab6992 at a 1/100 dilution staining CTGF from Mouse Tissue sections (Skin) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 12 hours and then detected using a Labeled AP polymer. The right side of image is regrown wounded epidermis and shows significant staining for CTGF.
This image is courtesy of an Abreview submitted by Martina Ziegenbein on 6 January 2006.
Immunohistochemistry (Frozen sections) - CTGF antibody (ab6992)
ab6992 at a 1/200 dilution staining CTGF in mouse liver tissue sections by Immunohistochemistry (frozen sections) incubated at +4°C for 9 hours. Fixed in formaldehyde, permeabilized using 0.2% Triton X-100. Blocked using 2% BSA for 30 minutes at 20°C. Secondary used at 1/200 dilution polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green).
This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence - CTGF antibody (ab6992)
ab6992 staining CTGF in Human 293FT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 25ºC. Samples were incubated with primary antibody (1/200 in PBS) for 2 hours at 25ºC. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - CTGF antibody (ab6992)
ab6692 staining CTGF in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25ºC. Samples were then incubated with primary antibody at 1/200 for 12 hours at 4ºC. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).
Image courtesy of an anonymous Abreview
Western blot - CTGF antibody (ab6992)
Anti-CTGF antibody (ab6992) at 1/5000 dilution (in PBS-T for 9 hours at 4°C) + Mouse brain whole cell lysate at 50 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
developed using the ECL technique
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Blocking Step: 5% Milk for 1 hour at 25°C
This image is courtesy of an anonymous Abreview
CTGF antibody for Western blot in Mouse (6992)
CTGF antibody for Western blot in Human (6992)
CTGF antibody for Immunocytochemistry/ Immunofluorescence in Mouse (6992)
CTGF antibody for IHC-Fr in Mouse (6992)
CTGF antibody for in Mouse (6992)
CTGF antibody for WB in Rat (6992)
CTGF antibody for Western blot in Mouse (6992)
CTGF antibody for IHC-Fr in Mouse (6992)
CTGF antibody for ICC/IF in Human (6992)
CTGF antibody for ICC/IF in Rat (6992)
CTGF antibody for Immunocytochemistry/ Immunofluorescence in Mouse (6992)
CTGF antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (6992)
12
