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Immunohistochemistry (Paraffin-embedded sections) - CTGF antibody (ab6992)
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Product Name
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CTGF antibody
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See all CTGF antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CTGF
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Immunogen
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Recombinant fragment, corresponding to amino acids 223-348 of Mouse CTGF.
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Reacts with
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Hu, Ms, Rat, Shp
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Predicted to react
(species key)
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Cow
(Due to sequence homology)
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Specificity
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Ueberham et al (Neuroscience, 2003)report "In human hippocampal protein extracts two distinct bands are detectable with a more intensive reactivity at about 37-38 kDa, representing the CTGF protein, and at about 42-44 kDa likely representing a glycosilated ... or a phosphorylated form of CTGF...".
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Tested applications
(see key)
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IHC-FoFr, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at an assay dependent dilution. PubMed 16100286.
IHC-F: 1/1500 (Tissue postfixed for 4 days at 4C in 4% paraformaldehyde in 0.1M PB, then immersed in 15% sucrose for 24hrs, 30% sucrose for 48hrs and sections were cut on a freezing microtome). Use Biotinylated secondary antibody then stextra-avidin-peroxidase chromogenic detection. (See Ueberham et al for details).
IHC-P: 1/400 - 1/800. (See Razzaque et al and Candido et al for details). Antigen retrieval can be avoided.
IHC-Fr: 1/500 (PMID 19299462).
WB: 1/5000. Can be blocked with CTGF (C-term) peptide (mouse). Block membrane in 4% BSA in Tris-buffered saline containing 0.05% Tween 20 (TBST).(see Fu M et al.).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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human hippocampal protein extracts (see Ueberham et al paper).
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Cellular localization
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Secreted
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Research areas
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Immunology >> Secreted Molecules >> CCN Family
Cardiovascular >> Angiogenesis >> Growth Factors >> Other
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Relevance
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CTGF (connective tissue growth factor) is a 38kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CTGF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CTGF antibody images, references, products related to ab6992 and other tools.
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CTGF antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - CTGF antibody (ab6992)
ab6992 at a 1/100 dilution staining CTGF from Mouse Tissue sections (Skin) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 12 hours and then detected using a Labeled AP polymer. The right side of image is regrown wounded epidermis and shows significant staining for CTGF. This image is courtesy of an Abreview submitted by Martina Ziegenbein on 6 January 2006.
See Abreview
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References for CTGF antibody (ab6992)
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This product has been used in: (two most recent references)
Shi-wen X et al. Rosiglitazone alleviates the persistent fibrotic phenotype of lesional skin scleroderma fibroblasts. Rheumatology (Oxford) 49:259-63 (2010). WB; Human. PubMed: 20007285
Gaultier A et al. Low-density lipoprotein receptor-related protein 1 is an essential receptor for myelin phagocytosis. J Cell Sci 122:1155-62 (2009). IHC-Fr; Human. PubMed: 19299462
See all 39 publication references for this product.
If you publish research using ab6992 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CTGF
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CTGF antibody - more information
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Customer reviews (feedback) regarding CTGF antibody |
Customer FAQs regarding CTGF antibody |
Protocols for CTGF antibody |
Price and availability of products related to CTGF antibody |
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CTGF antibody - related products:
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CTGF antibody - other tools:
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Contact Abcam with a Technical Enquiry about CTGF antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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