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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Overview
Product name
Anti-CTGF antibody
See all CTGF products (7) ...
Description
Rabbit polyclonal to CTGF
Specificity
Ueberham et al (Neuroscience, 2003)report "In human hippocampal protein extracts two distinct bands are detectable with a more intensive reactivity at about 37-38 kDa, representing the CTGF protein, and at about 42-44 kDa likely representing a glycosilated ... or a phosphorylated form of CTGF...".
Tested applications
IP, ICC/IF, WB, IHC-P, IHC-Fr, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Human
Predicted to work with
Cow
Immunogen
Recombinant fragment, corresponding to amino acids 223-348 of Mouse CTGF. Peptide available as ab7861.
Positive control
human hippocampal protein extracts (see Ueberham et al paper).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Secreted Molecules >> CCN Family
Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. (PubMed: 16100286)
ICC/IF: 1/200
WB: 1/5000(Block membrane in 4% BSA in Tris-buffered saline containing 0.05% Tween 20 (TBST). See Fu M et al.)
IHC-P: 1/400 - 1/800.Do not perform antigen retrieval.
IHC-Fr: Use at an assay dependent dilution. (See Ueberham et al.)
IHC-FoFr
IHC-FoFr: 1/1500IHC-FoFr: 1/1500
Target
Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Tissue specificity
Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted.
Target information above from: UniProt accessionP29279
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
see all
Database links
- Entrez Gene: 281103 Cow
- Entrez Gene: 1490 Human
- Entrez Gene: 14219 Mouse
- Entrez Gene: 64032 Rat
- Omim: 121009 Human
- SwissProt: O18739 Cow
- SwissProt: P29279 Human
- SwissProt: Q5M8T4 Human
see all
Anti-CTGF antibody images:
Immunohistochemistry (Paraffin-embedded sections) - CTGF antibody (ab6992)
ab6992 at a 1/100 dilution staining CTGF from Mouse Tissue sections (Skin) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 12 hours and then detected using a Labeled AP polymer. The right side of image is regrown wounded epidermis and shows significant staining for CTGF.
This image is courtesy of an Abreview submitted by Martina Ziegenbein on 6 January 2006.
Immunohistochemistry (Frozen sections) - CTGF antibody (ab6992)
ab6992 at a 1/200 dilution staining CTGF in mouse liver tissue sections by Immunohistochemistry (frozen sections) incubated at +4°C for 9 hours. Fixed in formaldehyde, permeabilized using 0.2% Triton X-100. Blocked using 2% BSA for 30 minutes at 20°C. Secondary used at 1/200 dilution polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green).
This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence - CTGF antibody (ab6992)
ab6992 staining CTGF in Human 293FT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in PBS) for 2 hours at 25°C. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - CTGF antibody (ab6992)
ab6692 staining CTGF in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).
Image courtesy of an anonymous Abreview
Western blot - CTGF antibody (ab6992)
Anti-CTGF antibody (ab6992) at 1/5000 dilution (in PBS-T for 9 hours at 4°C) + Mouse brain whole cell lysate at 50 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
developed using the ECL technique
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Blocking Step: 5% Milk for 1 hour at 25°C
This image is courtesy of an anonymous Abreview
References for Anti-CTGF antibody (ab6992)
This product has been referenced in:
- Candido Ret al. A breaker of advanced glycation end products attenuates diabetes-induced myocardial structural changes. Circ Res 92:785-92 (2003).Read more (PubMed: 12623881) »
- Razzaque MSet al. Role of connective tissue growth factor in the pathogenesis of conjunctival scarring in ocular cicatricial pemphigoid. Invest Ophthalmol Vis Sci 44:1998-2003 (2003).Read more (PubMed: 12714635) »
See all 43 publications for this product
Publishing research using ab6992? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"