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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Overview
Product name
CTGF protein (Active)
See all CTGF products (4) ...
Protein description
Recombinant fragment, corresponding to amino acids 253-350 of Human CTGF, a 11.2 kDa protein of 98 amino acid residues which exerts full heparin binding, cell adhesion, and mitogenic CTGF activity.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Purification notes
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Biological activity
Biological Activity : Determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1.0-2.0 µg/ml.
Form
Lyophilised
Storage instructions
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
Storage buffer
Spin down vial and reconstitute in distilled water for a concentration of 0.1 - 1.0 mg/ml.
Concentration
Concentration information loading...
Sequence notes
MGKKCIRTPK ISKPIKFELS GCTSMKTYRA KFCGVCTDGR CCTPHRTTTL PVEFKCPDGE VMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA
Research areas
Immunology >> Secreted Molecules >> CCN Family
Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
FuncS: Use at an assay dependent dilution. (Determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1.0-2.0 µg/ml. )
Protein info
Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Tissue specificity
Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted.
Target information above from: UniProt accessionP29279
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for CTGF protein (Active) (ab50044)
ab50044 has not yet been referenced specifically in any publications.
Publishing research using ab50044? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"