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Read our guarantee »Overview
Product name
CTIP2 (BCL11B) Human ELISA Kit
Specificity
Species– Human reactive.
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 8 | 9.6% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 24 | 13% |
Tests
1 x 96 test
Sample type
Cell culture supernatant, Tissue Extracts, Cell Lysate, Cell culture media
Assay type
Sandwich
Sensitivity
2 pg/ml
Recovery
92%
| Sample type | Average % | Range % |
|---|---|---|
| Cell culture supernatant | 90 | 80 - 105 |
| Serum | 94 | 78 - 117 |
Cross reactivity
Reacts with
Human
Product overview
ab123453 CTIP2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CTIP2 in cell and tissue lysates. The assay employs an antibody specific for Human CTIP2 coated onto 96-well plate strips.
Standards and samples are pipetted into the wells and the analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-CTIP2 primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Notes
Typical working ranges:
Whole Jurkat cell culture extract: 0.015 - 1 mg/ml
CTIP2 standard: 2 - 1000 ng/ml
Whole Jurkat cell culture extract: 0.015 - 1 mg/ml
CTIP2 standard: 2 - 1000 ng/ml
Store all components at 4°C. This kit is stable for at least 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Tested applications
Sandwich ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | 8209802 | 1 x 6ml |
| ab75607 - 10X CTIP2 Detector Antibody | 8209544 | 1 x 1ml |
| 10X HRP Label | 8206020 | 1 x 1ml |
| 20X Buffer | 8209552 | 1 x 20ml |
| CTIP2 Microplate | 8209543 | 1 unit |
| Extraction Buffer | 8209529 | 1 x 25ml |
| Human CTIP2 Standard | 1 vial | |
| TMB Development Solution | 8209803 | 1 x 12ml |
Function
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
Tissue specificity
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
Sequence similarities
Contains 6 C2H2-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9C0K0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B-cell CLL/lymphoma 11B
- B-cell lymphoma/leukemia 11B
- BC11B_HUMAN
see all
Database links
- Entrez Gene: 64919 Human
- SwissProt: Q9C0K0 Human
- Unigene: 709690 Human
Applications
Show applications key
Our Abpromise guarantee covers the use of ab123453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use at an assay dependent concentration.
CTIP2 (BCL11B) Human ELISA Kit images:
Sandwich ELISA - CTIP2 Human ELISA Kit (ab123453)
Example standard curve. A dilution series of recombinant CTIP2 in the working range of the assay.
Sandwich ELISA - CTIP2 Human ELISA Kit (ab123453)
Example of serially diluted endogenous CTIP2 expression in Jurkat cells.
Sandwich ELISA - CTIP2 Human ELISA Kit (ab123453)
Demonstration of the specificity of this ELISA. Endogenous expression of CTIP2 in Jurkat cells is higher than other non-lymphocyte cells such as Hela (human carcinoma) and NIH3T3 (mouse fibroblast), and C6 (rat glioma).
Sandwich ELISA - CTIP2 Human ELISA Kit (ab123453)
Demonstration of the specificity of this ELISA. CTIP2 expression is detectable in mouse brain lysate but not in rat brain lysate.
Sandwich ELISA - CTIP2 Human ELISA Kit (ab123453)
Demonstration of the specificity of the detector antibody used in this kit. (A) Western blot using the CTIP2 detector antibody at 1 µg/mL: Jurkat cell lysate and HeLa cell lysate, each were loaded at 16 µg/lane. (B) Analysis of CTIP2 staining in Jurkat cells by ICC: Left panel DAPI (red for contrast) with no primary antibody. Right panel CTIP2 detector primary antibody at 1 µg/mL (green), both samples contain 488 labeled secondary antibody. (C) Analysis of CTIP2 staining in Jurkat cells by Flow: Black unstained, red no primary and blue CTIP2 detector antibody at 1 µg/mL, red and blue include 488 dye labeled secondary.
Protocols
References for CTIP2 (BCL11B) Human ELISA Kit (ab123453)
ab123453 has not yet been referenced specifically in any publications.
Publishing research using ab123453? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"