CTLA 4 Mouse ELISA Kit (ab113346)
Overview
- Product nameCTLA 4 Mouse ELISA Kit
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 5 pg/ml
- Range4.92 pg/ml - 1200 pg/ml
- Recovery
96 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 76.34 67% - 89% Serum 107.3 101% - 114% Plasma 104.2 100% - 110% - Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse
- Product overview
ab113346 Mouse CTLA 4 (cytotoxic T-lymphocyte-associated molecule 4) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CTLA 4 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse CTLA 4 coated on a 96-well plate. Standards and samples are pipetted into the wells and CTLA 4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CTLA 4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CTLA 4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsSandwich ELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CTLA4 2 vials CTLA4 Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse CTLA4 Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab113346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
CTLA 4 Mouse ELISA Kit images
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Representative Standard CurveRepresentative Standard Curve using ab113346
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Representative Standard CurveRepresentative Standard Curve using ab113346
Protocols
References for CTLA 4 Mouse ELISA Kit (ab113346)
ab113346 has not yet been referenced specifically in any publications.
