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Flow Cytometry - CTLA4 antibody [1B8] (FITC) (ab24935)
![Flow Cytometry - CTLA4 antibody [1B8] (FITC) (ab24935)](/ps/datasheet/images/ab24935_1.jpg)
(enlarge)
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Product Name
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CTLA4 antibody [1B8] (FITC)
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See all CTLA4 antibodies (16)...
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Product type
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Primary antibodies
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Description
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Armenian Hamster monoclonal [1B8] to CTLA4 (FITC)
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Immunogen
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The details of the immunogen for this antibody are not available.
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Reacts with
(species key)
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Ms
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Specificity
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ab24935 recognises mouse CD152/CTLA-4
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Tested applications
(see key)
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Flow Cyt, FuncS, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 3µg for 106 cells.
FuncS: Use at an assay dependent dilution.
Note: ab24935 can be used for in vitro functional studies and for enhancement of tumor immunity.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein.
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Research areas
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Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Immune System Diseases >> Autoimmune
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
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Relevance
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CTLA4 has a critical role in T cell differentiation and contributes to the maintenance of peripheral T cell tolerance to self antigens. It exerts its major effects on T cell immune responses via regulation of the cell cycle and may be involved in T cell activation. It binds to B7-1 (CD80) and B7-2 (CD86), and it contains one immunoglobulin-like V type domain. It is widely expressed, with highest levels in lymphoid tissues.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 12477 (Mouse)
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Raised in
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Armenian Hamster
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Clonality
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Monoclonal
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Clone number
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1B8
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Conjugation
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Fluorescein
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C in the dark.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CTLA4 antibody [1B8] (FITC) is on this datasheet. But please do contact us if you would like any reassurance! |
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CTLA4 antibody [1B8] (FITC) images:
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 Flow Cytometry - CTLA4 antibody [1B8] (FITC) (ab24935)
CHO cells transfected with CTLA-4 were stained with
either hamster IgG FITC or ab24935 (3µg for 106 cells), following which large cells were gated and analyzed by flow cytometry.
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References for CTLA4 antibody [1B8] (FITC) (ab24935)
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This product has been used in:
Wang W et al. PD1 blockade reverses the suppression of melanoma antigen-specific CTL by CD4+ CD25(Hi) regulatory T cells. Int Immunol 21:1065-77 (2009). PubMed: 19651643
If you publish research using ab24935 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CTLA4
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CTLA4 antibody [1B8] (FITC) - more information
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Customer reviews (feedback) regarding CTLA4 antibody [1B8] (FITC) |
Customer FAQs regarding CTLA4 antibody [1B8] (FITC) |
Protocols for CTLA4 antibody [1B8] (FITC) |
Price and availability of products related to CTLA4 antibody [1B8] (FITC) |
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CTLA4 antibody [1B8] (FITC) - other tools:
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Contact Abcam with a Technical Enquiry about CTLA4 antibody [1B8] (FITC) |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Flow Cytometry - CTLA4 antibody [1B8] (FITC) (ab24935)](/index.html?datasheet=24935#ab24935_1.jpg)
