Overview

  • Product nameAnti-CUEDC2 antibodySee all CUEDC2 primary antibodies ...
  • Description
    Rabbit polyclonal to CUEDC2
  • Specificityab79036 will not cross-react with CUEDC1
  • Tested applicationsICC/IF, IP, WB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 19 amino acid synthetic peptide from near the carboxy terminus of human CUEDC2 (NP_076945).

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab79036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use a concentration of 20 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 32 kDa.
ELISA Use at an assay dependent dilution.

Target

Anti-CUEDC2 antibody images

  • Lane 1 : Anti-CUEDC2 antibody (ab79036) at 1 µg/ml
    Lane 2 : Anti-CUEDC2 antibody (ab79036) at 2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CUEDC2 antibody (ab79036)

ab79036 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Raw 264.7)
Loading amount 25 µg
Specification Raw 264.7
Treatment shRNA control or against CUEDC2
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
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Submitted Jul 31 2012

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Raw 264.7)
Total protein in input 200 µg
Specification Raw 264.7
Treatment 2hrs LPS
Immuno-precipitation step Protein G
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Submitted Jul 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"