Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)
Key features and details
- Mouse monoclonal [2A10] to Protein CASP - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Protein CASP antibody [2A10] - BSA and Azide free
See all Protein CASP primary antibodies -
Description
Mouse monoclonal [2A10] to Protein CASP - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment (GST-tag) corresponding to Human Protein CASP aa 500-650. NP_001904.2
Database link: Q13948 -
Positive control
- ICC/IF: HeLa cells. IHC-P: Human spleen tissue; human malignant lymphoma, diffuse large B tissue. Flow Cytometry: MCF7 cells. WB: Protein CASP transfected HEK-293T cell lysate. Recombinant Protein CASP protein.
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General notes
This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
2A10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab54583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 77.5 kDa.
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ICC/IF | (3) |
Use at an assay dependent concentration.
|
Sandwich ELISA |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 77.5 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
May be involved in intra-Golgi retrograde transport. -
Sequence similarities
Belongs to the CASP family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 1523 Human
- SwissProt: Q13948 Human
- Unigene: 191482 Human
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Alternative names
- CASP_HUMAN antibody
- CUX1 antibody
- Protein CASP antibody
Images
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Formalin-fixed, paraffin-embedded human spleen tissue stained for Protein CASP with ab54583 at 5 µg/ml in immunohistochemical analysis.
This image was generated using the ascites version of the product.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Protein CASP (green) using ab54583 at 10 µg/ml in ICC/IF.
This image was generated using the ascites version of the product.
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Overlay histogram showing MCF7 (human breast adenocarcinoma cell line) cells stained with ab54583 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3 M glycine to block non-specific protein-protein interactions followed by the antibody (ab54583, 1 µg/1 x 106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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All lanes : Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583) at 5 µg/ml
Lane 1 : Protein CASP transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 77.5 kDaThis image was generated using the ascites version of the product.
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Formalin-fixed, paraffin-embedded human malignant lymphoma, diffuse large B tissue stained for Protein CASP with ab54583 at 5 µg/ml in immunohistochemical analysis.
This image was generated using the ascites version of the product.
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Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583) at 5 µg/ml + Tagged recombinant Protein CASP protein
Predicted band size: 77.5 kDaThis antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Expected MW 38 kDa.
This image was generated using the ascites version of the product.
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Detection limit of ab54583 is approximately 0.03 ng/ml as a capture antibody.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (22)
ab54583 has been referenced in 22 publications.
- Papes F et al. Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content. Nat Commun 13:2387 (2022). PubMed: 35501322
- Schilders KAA et al. Identification of SOX2 Interacting Proteins in the Developing Mouse Lung With Potential Implications for Congenital Diaphragmatic Hernia. Front Pediatr 10:881287 (2022). PubMed: 35615634
- Rosebrock D et al. Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells. Nat Cell Biol 24:981-995 (2022). PubMed: 35697781
- Ye T et al. Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases. Commun Biol 4:195 (2021). PubMed: 33580208
- Pozo-Rodrigálvarez A et al. Hyperactive Behavior and Altered Brain Morphology in Adult Complement C3a Receptor Deficient Mice. Front Immunol 12:604812 (2021). PubMed: 33692783