Anti-CWC15 antibody (ab188345)
Key features and details
- Rabbit polyclonal to CWC15
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CWC15 antibody -
Description
Rabbit polyclonal to CWC15 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human CWC15 aa 1-100.
Database link: Q9P013 -
Positive control
- WB: Human cell line RT4, Human cell line U-251 MG sp, Human plasma (IgG/HSA depleted), Human liver tissue and Human tonsil tissue lysates. IHC-P: Human thyroid gland. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 27 kDa.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 27 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Belongs to the CWC15 family. -
Cellular localization
Nucleus -
Database links
- Entrez Gene: 51503 Human
- Entrez Gene: 66070 Mouse
- Entrez Gene: 300361 Rat
- SwissProt: Q9P013 Human
- SwissProt: Q9JHS9 Mouse
- SwissProt: Q5BJP2 Rat
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Alternative names
- CWC15 antibody
- AD-002 antibody
- C11orf5 antibody
see all
Images
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All lanes : Anti-CWC15 antibody (ab188345) at 1/100 dilution
Lane 2 : RT4 cell lysate
Lane 3 : U251 MG sp cell lysate
Lane 4 : Human plasma (IgG/HSA depleted)
Lane 5 : Human liver tissue lysate
Lane 6 : Human tonsil tissue lysate
Predicted band size: 27 kDa -
U-2 OS cells stained for CWC15 (green) using ab188345 at 2 µg/ml in ICC/IF.
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Paraffin embedded human thyroid gland tissue stained for CWC15 using ab188345 at 1/1000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab188345 has not yet been referenced specifically in any publications.