CX3CL1 Human ELISA Kit (ab100522)
- Product nameCX3CL1 Human ELISA KitSee all CX3CL1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 300 pg/ml
- Range0.25 ng/ml - 60 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.47 83% - 102% Serum 92.68 82% - 102% Plasma 93.54 83% - 103%
- Species reactivityReacts with: Human
- Product overview
ab100522, a Human CX3CL1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CX3CL1 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human CX3CL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CX3CL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CX3CL1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CX3CL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml CX3CL1 Microplate (12 strips x 8 wells) coated with anti-human CX3CL1 1 unit CX3CL1 Standard Human Recombinant 2 vials Detection Antibody CX3CL1: biotinylated anti-human CX3CL1 2 vials HRP-Streptavidin Concentrate 320x concentrated HRP-conjugated streptavidin 1 x 200µl Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- A 152E5.2AB030188ABCD 3
- ABCD3C X3 C motif chemokine 1C3XkineChemokine (C X3 C motif) ligand 1Chemokine C X3 C motif ligand 1Chemokine CX3C Motif Ligand 1CX3C membrane anchored chemokineCXC3CXC3CFKNFractalkineNeurotactinNTNNTTSCYD 1 SCYD1Small inducible cytokine D1Small inducible cytokine subfamily D (Cys X3 Cys), member 1Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) Small inducible cytokine subfamily D member 1
- SwissProt: P78423 Human
Our Abpromise guarantee covers the use of ab100522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CX3CL1 Human ELISA Kit (ab100522)
ab100522 has not yet been referenced specifically in any publications.