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Overview

  • Product nameCX3CL1 Human ELISA KitSee all CX3CL1 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 300 pg/ml
  • Range
    0.25 ng/ml - 60 ng/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.47 83% - 102%
    Serum 92.68 82% - 102%
    Plasma 93.54 83% - 103%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100522, a Human CX3CL1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CX3CL1 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human CX3CL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CX3CL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CX3CL1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CX3CL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     

     

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsELISA more details

Properties

  • RelevanceCX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.
    • Alternative names
        A 152E5.2AB030188ABCD 3
        ABCD3C X3 C motif chemokine 1C3XkineChemokine (C X3 C motif) ligand 1Chemokine C X3 C motif ligand 1Chemokine CX3C Motif Ligand 1CX3C membrane anchored chemokineCXC3CXC3CFKNFractalkineNeurotactinNTNNTTSCYD 1 SCYD1Small inducible cytokine D1Small inducible cytokine subfamily D (Cys X3 Cys), member 1Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) Small inducible cytokine subfamily D member 1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100522 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      CX3CL1 Human ELISA Kit images

      • ELISA - CX3CL1 Human ELISA Kit (ab100522)
        ELISA - CX3CL1 Human ELISA Kit (ab100522)

      Protocols

      References for CX3CL1 Human ELISA Kit (ab100522)

      ab100522 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"