Highly pure (>98%) recombinant hI-TAC (human Interferon-inducible T cell alpha chemoattractant)
This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A431
FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPBS, pH 7.4, no preservative, sterile filtered
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 10 kDa). To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use at an assay dependent concentration. To detect hI-TAC by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hI-TAC.
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hI-TAC (100 ng/ml), a concentration of 3 µg/ml of this antibody is required.
Use at an assay dependent concentration.
Use a concentration of 5 µg/ml.
FunctionChemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
Tissue specificityHigh levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
Small inducible cytokine subfamily B (Cys-X-Cys) member 11 antibody
Small inducible cytokine subfamily B (Cys-X-Cys) member 9B antibody
Small inducible cytokine subfamily B, member 11 antibody
Small inducible cytokine subfamily B, member 9B antibody
Small-inducible cytokine B11 antibody
Anti-CXCL11 antibody images
- Anti-CXCL11 antibody (ab9955)
ab9955 staining CXCL11 in human renal tumor with parenchyma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). The primary antibody was used at 0.125 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were acheived without antigen retrieval step.
Western blot - CXCL11 antibody (ab9955)
Anti-CXCL11 antibody (ab9955) at 1 µg/ml + Liver (Human) Tissue Lysate (ab29889) at 10 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody (ab9955)This image is courtesy of an Abreview submitted by R Berahovich, ChemoCentryx Inc, United States
ab9955 staining CXCL11 in Human glioblastoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer prior to blocking with a proprietary non-protein blocking solution for 5 minutes at 25°C. The primary antibody was diluted 1/500 and incubated with the sample for 30 minutes. A biotin-conjugated goat anti-rabbit IgG was used as the secondary antibody, diluted 1/500.
ICC/IF image of ab9955 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9955 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-CXCL11 antibody (ab9955)
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