Overview

  • Product nameAnti-CXCL11 antibodySee all CXCL11 primary antibodies ...
  • Description
    Rabbit polyclonal to CXCL11
  • Tested applicationsWB, ELISA, Neutralising, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hI-TAC (human Interferon-inducible T cell alpha chemoattractant)

  • Positive control
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A431

Properties

Applications

Our Abpromise guarantee covers the use of ab9955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 14 kDa (predicted molecular weight: 10 kDa). To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent dilution. To detect hI-TAC by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hI-TAC.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hI-TAC (100 ng/ml), a concentration of 3 µg/ml of this antibody is required.
IHC-P Use at an assay dependent dilution.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionChemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
  • Tissue specificityHigh levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • b R1 antibody
    • b-R1 antibody
    • Beta-R1 antibody
    • betaR1 antibody
    • C-X-C motif chemokine 11 antibody
    • Chemokine (C-X-C motif) ligand 11 antibody
    • Chemokine C-X-C motif ligand 11 antibody
    • CXC11 antibody
    • CXCL11 antibody
    • CXL11_HUMAN antibody
    • H174 antibody
    • I TAC antibody
    • I-TAC antibody
    • Interferon gamma inducible protein 9 antibody
    • Interferon gamma-inducible protein 9 antibody
    • Interferon inducible T cell alpha chemoattractant antibody
    • Interferon-inducible T-cell a chemoattractant I-TAC antibody
    • Interferon-inducible T-cell alpha chemoattractant antibody
    • IP 9 antibody
    • IP-9 antibody
    • IP9 antibody
    • ITAC antibody
    • MGC102770 antibody
    • SCYB11 antibody
    • SCYB9B antibody
    • small inducible cytokine B11 antibody
    • small inducible cytokine subfamily B (Cys-X-Cys) member 11 antibody
    • small inducible cytokine subfamily B (Cys-X-Cys) member 9B antibody
    • Small inducible cytokine subfamily B, member 11 antibody
    • Small inducible cytokine subfamily B, member 9B antibody
    • Small-inducible cytokine B11 antibody
    see all

Anti-CXCL11 antibody images

  • ab9955 staining CXCL11 in human renal tumor with parenchyma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).  The primary antibody was used at 0.125 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were acheived without antigen retrieval step.

  • Anti-CXCL11 antibody (ab9955) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 10 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa,45 kDa,49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ab9955 staining CXCL11 in Human glioblastoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer prior to blocking with a proprietary non-protein blocking solution for 5 minutes at 25°C. The primary antibody was diluted 1/500 and incubated with the sample for 30 minutes. A biotin-conjugated goat anti-rabbit IgG was used as the secondary antibody, diluted 1/500.

    See Abreview

  • ICC/IF image of ab9955 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9955 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CXCL11 antibody (ab9955)

ab9955 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Glioblastoma)
Specification Glioblastoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step (agent) for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

Submitted Nov 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"