Anti-CXCL11 antibody (ab9955)

Overview

• Product nameAnti-CXCL11 antibodySee all CXCL11 primary antibodies ...
• Description
  Rabbit polyclonal to CXCL11
• Tested applicationsWB, ELISA, Neutralising, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Highly pure (>98%) recombinant hI-TAC (human Interferon-inducible T cell alpha chemoattractant)

• Positive controlThis antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A431

Properties

• FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS, pH 7.4, no preservative, sterile filtered
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Immunology
  • Innate Immunity
  • Chemokines
  • Alpha Chemokines (CXC)

  • Immunology
  • Immune System Diseases
  • Autoimmune

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

Applications

Target

• FunctionChemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
• Tissue specificityHigh levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
• Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
• Cellular localizationSecreted.

Target information above from: UniProt accession O14625 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6373 Human
  • Omim: 604852 Human
  • SwissProt: O14625 Human
  • Unigene: 632592 Human

• Alternative names
  b R1 antibodyb-R1 antibodyBeta-R1 antibody

  betaR1 antibodyC-X-C motif chemokine 11 antibodyChemokine (C-X-C motif) ligand 11 antibodyChemokine C-X-C motif ligand 11 antibodyCXC11 antibodyCXCL11 antibodyCXL11_HUMAN antibodyH174 antibodyI TAC antibodyI-TAC antibodyInterferon gamma inducible protein 9 antibodyInterferon gamma-inducible protein 9 antibodyInterferon inducible T cell alpha chemoattractant antibodyInterferon-inducible T-cell a chemoattractant I-TAC antibodyInterferon-inducible T-cell alpha chemoattractant antibodyIP 9 antibodyIP-9 antibodyIP9 antibodyITAC antibodyMGC102770 antibodySCYB11 antibodySCYB9B antibodysmall inducible cytokine B11 antibodysmall inducible cytokine subfamily B (Cys-X-Cys) member 11 antibodysmall inducible cytokine subfamily B (Cys-X-Cys) member 9B antibodySmall inducible cytokine subfamily B, member 11 antibodySmall inducible cytokine subfamily B, member 9B antibodySmall-inducible cytokine B11 antibody

  see all

Anti-CXCL11 antibody images

• 

  - Anti-CXCL11 antibody (ab9955)

  ab9955 staining CXCL11 in human renal tumor with parenchyma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).  The primary antibody was used at 0.125 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were acheived without antigen retrieval step.

• 

  Western blot - CXCL11 antibody (ab9955)
  Anti-CXCL11 antibody (ab9955) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 10 kDa
  Observed band size : 14 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 31 kDa,45 kDa,49 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 30 seconds
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody (ab9955)This image is courtesy of an Abreview submitted by R Berahovich, ChemoCentryx Inc, United States
  ab9955 staining CXCL11 in Human glioblastoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer prior to blocking with a proprietary non-protein blocking solution for 5 minutes at 25°C. The primary antibody was diluted 1/500 and incubated with the sample for 30 minutes. A biotin-conjugated goat anti-rabbit IgG was used as the secondary antibody, diluted 1/500.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CXCL11 antibody (ab9955)

  ICC/IF image of ab9955 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9955 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.