Anti-CXCR3 antibody [2Ar1] (ab64714)

  Immunocytochemistry/ Immunofluorescence - CXCR3 antibody [2Ar1] (ab64714)

ab64714 staining CXCR3 in primary cultured HUVEC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/400 in PBS + 2.5% serum) for 16 hours at 4°C. An Alexa Fluor^®488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Bing Wang

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR3 antibody [2Ar1] (ab64714)

ab64714 staining CXCR3 in pig uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to enzymatic antigen retrieval prior to blocking with 10% serum for 20 minutes at 24°C. The primary antibody was diluted 1/200 and incubated with the sample for 30 minutes at 24°C. A biotin-conjugated ABC kit was used to detect the antibody.

This image is courtesy of an Abreview submitted by Frank White

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR3 antibody [2Ar1] (ab64714)

ab64714 staining CXCR3 in Human glioblastoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer prior to blocking with a proprietary non-protein reagent for 5 minutes at 25°C. The primary antibody was diluted 1/2000 and incubated with the sample for 30 minutes. A biotin-conjugated goat anti-mouse IgG was used as the secondary antibody.

This image is courtesy of an Abreview submitted by R Berahovich, ChemoCentryx Inc, United States

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