Anti-CXCR3 antibody [49801.111] (ab10402)

Product name

Anti-CXCR3 antibody [49801.111]
See all CXCR3 products (7) ...

Description

Mouse monoclonal [49801.111] to CXCR3

Specificity

This product reacts with CXCR3 transfected cells and not with the parent cell line by flow cytometry. The antibody shows no cross-reactivity with human CXCR1, CXCR2 and CXCR4.

Tested applications

Flow Cytmore details

Cross reactivity

Reacts with

Human

Immunogen

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS
Endotoxin level is < 10 ng per mg antibody as determined by the LAL method.

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

The antibody is purified from ascites fluid using protein G chromatography.

Primary antibody notes

Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX₃CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX₃CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CXCR3, also known as the IP10/ Mig receptor, shares approximately 40% protein sequence identity with CXCR1 and CXCR2, and 34. 2 -36.9% amino acid sequence identity with the five known CC chemokine receptors 1-5. CXCR3 is highly expressed by IL2 activated T lymphocytes (Th1), but not by resting T lymphocytes, B lymphocytes, monocytes or granulocytes. CXCR3 binds IP10 and Mig (but not PF4) with high affinity and mediates Ca²⁺ mobilization and chemotaxis. CXCR3 does not bind any of the CXC chemokines containing the ELR motif.

Clonality

Monoclonal

Clone number

49801.111

Myeloma

NS0

Isotype

IgG1

Research areas

Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Other
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)