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Products:Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)
Overview
Product name
Anti-CXCR3 antibody
See all CXCR3 products (7) ...
Description
Rabbit polyclonal to CXCR3
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CXCR3.
(Peptide available as ab74828.)
Positive control
This antibody gave a positive signal in the following human tissue lysates: Colon, Small Intestine, Lymph Node and Heart
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Other
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)
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Western blot - CXCR3 antibody (ab71864)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 70 kDa (predicted molecular weight: 41 kDa).
Target
Function
Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
Isoform 3 mediates the activity of CXCL11.
Tissue specificity
Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP49682
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C-X-C chemokine receptor type 3 antibody
- CD 183 antibody
- CD183 antibody
see all
Database links
- Entrez Gene: 2833 Human
- Entrez Gene: 12766 Mouse
- Entrez Gene: 84475 Rat
- Omim: 300574 Human
- SwissProt: P49682 Human
- SwissProt: O88410 Mouse
- SwissProt: Q9JII9 Rat
- Unigene: 198252 Human
see all
Anti-CXCR3 antibody images:
Western blot - CXCR3 antibody (ab71864)
All lanes : Anti-CXCR3 antibody (ab71864) at 1 µg/ml
Lane 1 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)
Lane 2 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)
Lane 3 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)
Lane 4 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 41 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
Our observed 70kDa band is supported by the following publications: http://www.jbc.org/content/279/29/30219.long and http://www.jimmunol.org/cgi/content/full/173/10/6234
References for Anti-CXCR3 antibody (ab71864)
ab71864 has not yet been referenced specifically in any publications.
Publishing research using ab71864? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"