CXCR4 antibody - Hematopoietic Stem Cell Marker (ab1670)

Product Name 

CXCR4 antibody - Hematopoietic Stem Cell Marker

Product type 

Primary antibodies

Description 

Goat polyclonal to CXCR4 - Hematopoietic Stem Cell Marker

Immunogen 

Synthetic peptide: YSEEVGSGDYDSNKEPCFRDENVHFNR, corresponding to N terminal amino acids 14-40 of Mouse CXCR4
BLAST 'YSEEVGSGDYDSNKEPCFRDENVHFNR' with or

Reacts with 
(species key)

Hu, Ms

Specificity 

This antibody binds to CXCR 4 receptor on mouse spleen leukocytes as determined by immunocytochemistry.

Tested applications 
(see key)

ELISA, Flow Cyt, ICC, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/50000.
Flow Cyt: Use at an assay dependent dilution (PMID 18369102).
ICC: 1/300.
IHC-P: 1/300.
WB: 1/1000.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse spleen cells

Cellular localization 

Integral membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)

Relevance 

CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T cell tropic strains of HIV1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual tropic strains of HIV1 and mediates CD4 independent infection by HIV2. The a chemokine SDF1 is the ligand for CXCR4 and prevents infection by T tropic HIV1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV1 and HIV2 fusion and infection of human target cells. The amino terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites. CXCR4 is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS, 1mg/ml BSA, 0.1% sodium azide, pH7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for CXCR4 antibody - Hematopoietic Stem Cell Marker (ab1670)

  This product has been used in: (two most recent references)

Grundler R et al. Dissection of PIM serine/threonine kinases in FLT3-ITD-induced leukemogenesis reveals PIM1 as regulator of CXCL12-CXCR4-mediated homing and migration. J Exp Med 206:1957-70 (2009). WB; Human. PubMed: 19687226

Nelson TJ et al. CXCR4+/FLK-1+ biomarkers select a cardiopoietic lineage from embryonic stem cells. Stem Cells 26:1464-73 (2008). Flow Cyt; Mouse. PubMed: 18369102

See all 5 publication references for this product.


If you publish research using ab1670 please let us know so that we can cite the reference on this datasheet.

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