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Western blot - CXCR4 antibody (ab2074)
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Immunocytochemistry - CXCR4 antibody (ab2074)
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Immunocytochemistry/ Immunofluorescence - CXCR4 antibody (ab2074)
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Product Name
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CXCR4 antibody
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See all CXCR4 antibodies (16)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CXCR4
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Immunogen
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Synthetic peptide, corresponding to N terminal amino acids 1-14 of Human CXCR4(Peptide available as ab8126.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Epitope
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MEGISIYTSDNYTE
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at a concentration of 2 - 10 µg/ml.
Flow Cyt: 1/50 - 1/100.
IHC-P: 1/50 (see review. Unfortunately we have no further information regarding this application).
IHC-Fr: 1/2000 (see review). Fix with 4% paraformaldehyde. Permeabilise with methanol. Amplification necessary.
ICC-IF: 1/100 (see Abreview)
IP: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 39 kDa). Can be blocked with CXCR4 (N-term) peptide (human). To block use 5% non-fat dry milk in TBS to block for 2 hours at RT or overnight at 4°C.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate.
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Cellular localization
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Integral membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)
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Relevance
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CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T cell tropic strains of HIV1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV1 and mediates CD4 independent infection by HIV2. The a chemokine SDF1 is the ligand for CXCR4 and prevents infection by T tropic HIV1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV1 and HIV2 fusion and infection of human target cells. The amino terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites. CXCR4 is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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PBS with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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HIV and chemokine receptor.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CXCR4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CXCR4 antibody images, references, products related to ab2074 and other tools.
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CXCR4 antibody images:
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 Western blot - CXCR4 antibody (ab2074)
CXCR4 antibody (ab2074) at 1/2000 dilution + HeLa whole cell lysate
Predicted band size : 39 kDa
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Immunocytochemistry - CXCR4 antibody (ab2074)
Immunocytochemistry of CXCR4 in HeLa cells with CXCR4 antibody ab2074 at 2 µg/ml.
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Immunocytochemistry/ Immunofluorescence - CXCR4 antibody (ab2074)
ab2074 at 1/100 staining differentiated rat skeletal myocytes by ICC/IF. The cells were 2% paraformaldehyde fixed for 15 minutes and then incubated cells with ab2074 overnight at 4°C. The image demonstrates CXCR4 expressing skeletal myocytes (green-cytoplasmic and cell membrane localization) with DAPI (blue-nuclear counter stain) [Right panel] and DIC (phase) image of the same skeletal myocytes ([left panel]. This image is courtesy of an Abreview submitted by Dr Mal Niladri
See Abreview
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Immunocytochemistry/ Immunofluorescence - CXCR4 antibody (ab2074)
ab2074 staining CXCR4 expressing cells (of mesenchymal origin) by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incubation with the antibody for 1 hour. A FITC conjugated swine anti-rabbit antibody was used as the secondary. Nuclei were counterstained with DAPI. This image is courtesy of an anonymous Abreview
See Abreview
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Flow Cytometry - CXCR4 antibody (ab2074)
ab2074 staining cells from human B cell lymphoma by flow cytometry. Cells were cultured and prepared in FACS buffer. The primary antibody was diluted 1/50 and incubated with the sample for 20 minutes at 4°C. A FITC conjugated swine anti-rabbit antibody was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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References for CXCR4 antibody (ab2074)
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This product has been used in: (two most recent references)
Campbell GR et al. HIV-1 clade B Tat, but not clade C Tat, increases X4 HIV-1 entry into resting but not activated CD4+ T cells. J Biol Chem 285:1681-91 (2010). WB; Human. PubMed: 19917610
Hong JS et al. CXCR-4 knockdown by small interfering RNA inhibits cell proliferation and invasion of oral squamous cell carcinoma cells. J Oral Pathol Med 38:214-9 (2009). WB. PubMed: 18624931
See all 19 publication references for this product.
If you publish research using ab2074 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CXCR4
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CXCR4 antibody - more information
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Customer reviews (feedback) regarding CXCR4 antibody |
Customer FAQs regarding CXCR4 antibody |
Protocols for CXCR4 antibody |
Price and availability of products related to CXCR4 antibody |
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CXCR4 antibody - related products:
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CXCR4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CXCR4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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