CXCR4 antibody (ab7199)

Product Name 

CXCR4 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to CXCR4

Immunogen 

Recombinant full length protein (Rat).

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: 1/500. (PMID 16841089)
IHC-P: Use at an assay dependent concentration.
WB: 1/2000.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Integral membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)

Relevance 

CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T cell tropic strains of HIV1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual tropic strains of HIV1 and mediates CD4 independent infection by HIV2. The a chemokine SDF1 is the ligand for CXCR4 and prevents infection by T tropic HIV1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV1 and HIV2 fusion and infection of human target cells. The amino terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites. CXCR4 is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for CXCR4 antibody (ab7199)

  This product has been used in: (two most recent references)

Abe H et al. Role of the CXCL12/CXCR4 axis in milky spots of rats bearing ascitic-type hepatoma. Anat Sci Int 84:226-36 (2009). WB, ICC/IF, IHC-P; Rat. PubMed: 19340530

Suuronen EJ et al. An acellular matrix-bound ligand enhances the mobilization, recruitment and therapeutic effects of circulating progenitor cells in a hindlimb ischemia model. FASEB J 23:1447-58 (2009). Flow Cyt; Rat. PubMed: 19136616

See all 9 publication references for this product.


If you publish research using ab7199 please let us know so that we can cite the reference on this datasheet.

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