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Western blot - CXCR4 antibody (ab77909)
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Immunocytochemistry/ Immunofluorescence - CXCR4 antibody (ab77909)
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Product Name
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CXCR4 antibody
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See all CXCR4 antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CXCR4
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Immunogen
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A synthesized peptide derived from the C terminal of human CXCR4.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Specificity
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This antibody detects endogenous levels of total CXCR4 protein.
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 40 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A549 cells and extracts from K562 cells.
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Cellular localization
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Integral membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)
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Relevance
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CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T cell tropic strains of HIV1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual tropic strains of HIV1 and mediates CD4 independent infection by HIV2. The a chemokine SDF1 is the ligand for CXCR4 and prevents infection by T tropic HIV1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV1 and HIV2 fusion and infection of human target cells. The amino terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites. CXCR4 is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CXCR4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CXCR4 antibody images, references, products related to ab77909 and other tools.
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CXCR4 antibody images:
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 Western blot - CXCR4 antibody (ab77909)
All lanes : CXCR4 antibody (ab77909) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cell with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 40 kDa
Observed band size : 40 kDa
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Immunocytochemistry/ Immunofluorescence - CXCR4 antibody (ab77909)
Immunofluorescence analysis of A549 cells using ab77909 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
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Search PubMed (MEDLINE) for references to CXCR4
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CXCR4 antibody - more information
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Customer reviews (feedback) regarding CXCR4 antibody |
Customer FAQs regarding CXCR4 antibody |
Protocols for CXCR4 antibody |
Price and availability of products related to CXCR4 antibody |
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CXCR4 antibody - related products:
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CXCR4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CXCR4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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