Overview

  • Product nameAnti-CYFIP2 antibody
    See all CYFIP2 primary antibodies
  • Description
    Rabbit polyclonal to CYFIP2
  • Conjugation notesab95969 was purified by antigen affinity chromatography.
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 346-610 of Human CYFIP2 (UniProt ID: Q96F07 isoform 2; NP_055191).

  • Positive control
    • WB: MOLT4 whole cell lysate; Raji cell lysate IHC-P: NCIN87 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab95969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 146 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 24658146

Target

  • RelevanceCYFIP2 is involved in T cell adhesion and p53 dependent induction of apoptosis. It does not bind RNA but is up regulated significantly in CD4+ T lymphocytes from patients with multiple sclerosis. There are 2 isoforms produced by alternative splicing.
  • Cellular localizationCytoplasm perinuclear region. Cell junction, synapse, synaptosome. Treatment with leptomycin-B triggers translocation to the nucleus.
  • Database links
  • Alternative names
    • CYFP2 antibody
    • cytoplasmic FMR1 interacting protein 2 antibody
    • KIAA1168 antibody
    • p53 inducible protein antibody
    • p53-inducible protein 121 antibody
    • PIR121 antibody
    see all

Anti-CYFIP2 antibody images

  • Anti-CYFIP2 antibody (ab95969) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 146 kDa
  • ab95969, at a 1/100 dilution, staining CYFIP2 in paraffin embedded NCIN87 xenograft by Immunohistochemistry.

References for Anti-CYFIP2 antibody (ab95969)

This product has been referenced in:
  • Taha MS  et al. Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin. PLoS One 9:e91465 (2014). ICC/IF ; Human . Read more (PubMed: 24658146) »
  • Mongroo PS  et al. IMP-1 displays cross-talk with K-Ras and modulates colon cancer cell survival through the novel proapoptotic protein CYFIP2. Cancer Res 71:2172-82 (2011). WB ; Human . Read more (PubMed: 21252116) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. As a general guidance, I can make some suggestions in order to improve the results from this antibody. As a lysis buffer I would suggest using RIPA (radio immuno precipitation assay) buffer. You can prepare it following the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"