Overview

  • Product nameAnti-CYP2A13 antibodySee all CYP2A13 primary antibodies ...
  • Description
    Rabbit polyclonal to CYP2A13
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Guinea pig, Cow, Pig
  • Immunogen

    Synthetic peptide: SVLRDPRFFS NPRDFNPQHF LDKKGQFKKS DAFVPFSIGK RYCFGEGLAR, corresponding to C terminal amino acids 397-446 of Human CYP2A13

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab58740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

Target

  • FunctionExhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N-dimethylaniline, 2'-methoxyacetophenone, N-nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity.
  • Tissue specificityExpressed in liver and a number of extrahepatic tissues, including nasal mucosa, lung, trachea, brain, mammary gland, prostate, testis, and uterus, but not in heart, kidney, bone marrow, colon, small intestine, spleen, stomach, thymus, or skeletal muscle.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CP2AD_HUMAN antibody
    • CPAD antibody
    • CYP2A antibody
    • CYP2A13 antibody
    • CYPIIA13 antibody
    • Cytochrome P450 2A13 antibody
    • CYTOCHROME P450, SUBFAMILY IIA, POLYPEPTIDE 13 antibody
    see all

Anti-CYP2A13 antibody images

  • Anti-CYP2A13 antibody (ab58740) at 5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.

    Predicted band size : 57 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)

References for Anti-CYP2A13 antibody (ab58740)

ab58740 has not yet been referenced specifically in any publications.

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