You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-CYP2A13 antibodySee all CYP2A13 primary antibodies ...
  • Description
    Rabbit polyclonal to CYP2A13
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide: SVLRDPRFFS NPRDFNPQHF LDKKGQFKKS DAFVPFSIGK RYCFGEGLAR, corresponding to C terminal amino acids 397-446 of Human CYP2A13

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab58740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1:312500
    WB: Use at a concentration of 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionExhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N-dimethylaniline, 2'-methoxyacetophenone, N-nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity.
    • Tissue specificityExpressed in liver and a number of extrahepatic tissues, including nasal mucosa, lung, trachea, brain, mammary gland, prostate, testis, and uterus, but not in heart, kidney, bone marrow, colon, small intestine, spleen, stomach, thymus, or skeletal muscle.
    • Sequence similaritiesBelongs to the cytochrome P450 family.
    • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.

      • Target information above from: UniProt accession Q16696 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CP2AD_HUMAN antibodyCPAD antibodyCYP2A antibody
          CYP2A13 antibodyCYPIIA13 antibodyCytochrome P450 2A13 antibodyCYTOCHROME P450, SUBFAMILY IIA, POLYPEPTIDE 13 antibody
        see all

      Anti-CYP2A13 antibody images

      • Western blot - CYP2A13 antibody (ab58740)
        Western blot - CYP2A13 antibody (ab58740)
        Anti-CYP2A13 antibody (ab58740) at 5 µg/ml + HepG2 cell lysate at 10 µg

        Secondary
        HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.

        Predicted band size : 57 kDa
        Observed band size : 54 kDa (why is the actual band size different from the predicted?)

      References for Anti-CYP2A13 antibody (ab58740)

      ab58740 has not yet been referenced specifically in any publications.

      Publishing research using ab58740 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab58740 Submit a Question for ab58740

      There are currently no Abreviews or Questions for ab58740.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"