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Products:Signal Transduction >> Metabolism >> Lipid metabolism
Overview
Product name
Anti-CYP7B1 antibody
See all CYP7B1 products (3) ...
Description
Goat polyclonal to CYP7B1
Tested applications
IHC-P, WBmore details
Cross reactivity
Predicted to work with
Cow, Human
Immunogen
Synthetic peptide: YPDSDVLFRYKVKS, corresponding to C terminal amino acids 493-506 of Human CYP7B1.
YPDSDVLFRY KVKS
Positive control
Human Breast tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CYP7B1 antibody (ab19043)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab19043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 3 - 6 µg/ml.
WB: Use at an assay dependent dilution. (PubMed: 19729835)
Target
Relevance
The CYP7B1 protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme is likely to play a minor role in total bile acid synthesis, and may also be involved in the development of neurosteroid metabolism, atherosclerosis and sex hormone synthesis, and is a member of the cytochrome P450 superfamily of enzymes.
Cellular localization
Endoplasmic reticulum
Alternative names
- 25 hydroxycholesterol 7 alpha hydroxylase antibody
- CP7B antibody
- Cytochrome P450 7B1 antibody
see all
Database links
- Entrez Gene: 9420 Human
- Omim: 603711 Human
- SwissProt: O75881 Human
Anti-CYP7B1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CYP7B1 antibody (ab19043)
ab19043 at 4µg/ml, staining CYP7B1 in Human Breast tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with pH 6 citrate buffer was performed, and HRP-staining was used.
References for Anti-CYP7B1 antibody (ab19043)
This product has been referenced in:
- Leuenberger Net al. Sumoylated PPARalpha mediates sex-specific gene repression and protects the liver from estrogen-induced toxicity in mice. J Clin Invest : (2009). WB; Mouse.Read more (PubMed: 19729835) »
See 1 publication for this product
Publishing research using ab19043? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"