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Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2-ß/?/d (Ab-287) antibody at 1/50-1/100 dilution.
All lanes : Anti-CaMK2 beta gamma delta antibody (ab79359) at 1/500 dilution
Lane 1 : Rat brain cell extract
Lane 2 : Rat brain cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 73 kDa
Observed band size : ~73 kDa (why is the actual band size different from the predicted?)
Additional bands at : ~50 kDa. We are unsure as to the identity of these extra bands.
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