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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Overview
Product name
Anti-CaMK2 beta gamma delta antibody
Description
Rabbit polyclonal to CaMK2 beta gamma delta
Specificity
Antibody detects endogenous levels of total CaMK2-beta/gamma/delta protein.
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized non-phosphopeptide derived from human CaMK2-beta/gamma/delta around the phosphorylation site of threonine 287 (Q-E-TP-V-E).
Positive control
Human brain tissue and rat brain cell extract
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
WB: 1/500 - 1/1000.Predicted molecular weight: 73 kDa.
IHC-P: 1/50 - 1/100.
Target
Relevance
The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- Calcium/calmodulin-dependent protein antibody
- CaM kinase II beta subunit antibody
- CaM-kinase II beta chain antibody
see all
Database links
- Entrez Gene: 816 Human
- Entrez Gene: 817 Human
- Entrez Gene: 818 Human
- Entrez Gene: 108058 Mouse
- Entrez Gene: 12323 Mouse
- Entrez Gene: 12325 Mouse
- Entrez Gene: 171140 Rat
- Entrez Gene: 24245 Rat
see all
Anti-CaMK2 beta gamma delta antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CaMK2 beta gamma delta antibody (ab79359)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2-ß/?/d (Ab-287) antibody at 1/50-1/100 dilution.
Western blot - CaMK2 beta gamma delta antibody (ab79359)
All lanes : Anti-CaMK2 beta gamma delta antibody (ab79359) at 1/500 dilution
Lane 1 : Rat brain cell extract
Lane 2 : Rat brain cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 73 kDa
Observed band size : ~73 kDa (why is the actual band size different from the predicted?)
Additional bands at : ~50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-CaMK2 beta gamma delta antibody (ab79359)
ab79359 has not yet been referenced specifically in any publications.
Publishing research using ab79359? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"