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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CaMK2 beta gamma delta antibody (ab79359)
(enlarge)
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Western blot - CaMK2 beta gamma delta antibody (ab79359)
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Product Name
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CaMK2 beta gamma delta antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CaMK2 beta gamma delta
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Immunogen
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Synthesized non-phosphopeptide derived from human CaMK2-beta/gamma/delta around the phosphorylation site of threonine 287 (Q-E-TP-V-E).
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Specificity
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Antibody detects endogenous levels of total CaMK2-beta/gamma/delta protein.
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human brain tissue and rat brain cell extract
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
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Relevance
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The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene.
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CaMK2 beta gamma delta antibody (ab79359) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CaMK2 beta gamma delta antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CaMK2 beta gamma delta antibody images, references, products related to ab79359 and other tools.
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CaMK2 beta gamma delta antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CaMK2 beta gamma delta antibody (ab79359)
Immunohistochemistry analysis of paraffin-embedded
human brain tissue using CaMK2-β/γ/δ (Ab-287) antibody at 1/50-1/100 dilution.
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Western blot - CaMK2 beta gamma delta antibody (ab79359)
All lanes : CaMK2 beta gamma delta antibody (ab79359) at 1/500 dilution
Lane 1 : Rat brain cell extract
Lane 2 : Rat brain cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 73 kDa
Observed band size : ~73 kDa (why is the actual band size different from the predicted?)
Additional bands at : ~50 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to CaMK2 beta gamma delta
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CaMK2 beta gamma delta antibody - more information
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Customer reviews (feedback) regarding CaMK2 beta gamma delta antibody |
Customer FAQs regarding CaMK2 beta gamma delta antibody |
Protocols for CaMK2 beta gamma delta antibody |
Price and availability of products related to CaMK2 beta gamma delta antibody |
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CaMK2 beta gamma delta antibody - related products:
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CaMK2 beta gamma delta antibody - other tools:
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Contact Abcam with a Technical Enquiry about CaMK2 beta gamma delta antibody |
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