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Anti-CaMKI antibody [EPR2217Y] (ab68234)

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Overview

Product name

Anti-CaMKI antibody [EPR2217Y]
See all CaMKI products (9) ...

Description

Rabbit monoclonal [EPR2217Y] to CaMKI

Tested applications

WB, IHC-P, ICCmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A specific peptide corresponding to residues on the N-terminus of human CaMKI.

Positive control

SH-SY5Y lysate, human, mouse and rat brain lysate and human kidney tissue sections.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR2217Y

Isotype

IgG

Research areas

Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK

  • Western blot - CaMKI antibody [EPR2217Y] (ab68234)Western blot - CaMKI antibody [EPR2217Y] (ab68234) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CaMKI antibody [EPR2217Y] (ab68234)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CaMKI antibody [EPR2217Y] (ab68234) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab68234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/5000 - 1/10,000.De...Read more →

    WB: 1/5000 - 1/10,000.Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.

    IHC-P: 1/100 - 1/250.

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

Application notes

Is unsuitable for ,Flow Cyt or IP.

Target

Relevance

Calcium / calmodulin-dependent kinases (CaMKs) are a subfamily of the serine/threonine protein kinase family that includes CaMKI, CaMKII, and CaMKIV. There have been identified multiple substrates for CaMKI, including synapsin 1, synapsin 2, CREB, and CFTR, and its activation is dependent upon phosphorylation of Thr 177 by CaMK kinase (CaMKK). Studies suggest that CaMKI plays an important role in retinal function and its expression is regulated by light stimulation.

Cellular localization

Cytoplasmic and Nuclear

Alternative names

  • Calcium / calmodulin dependent protein kinase 1 antibody
  • Calcium / calmodulin dependent protein kinase I antibody
  • Calcium / calmodulin dependent protein kinase type 1 antibody
  • Calcium/calmodulin dependent protein kinase 1 antibody
  • Calcium/calmodulin dependent protein kinase I antibody
  • Calcium/calmodulin dependent protein kinase type 1 antibody
  • CaM K1 antibody
  • CaM KI antibody
  • CaM kinase 1 alpha antibody
  • CAM kinase 1 antibody
  • CaM kinase I alpha antibody
  • CAM kinase I antibody
  • CaMK 1 antibody
  • CAMK I antibody
  • CaMK1 alpha antibody
  • CaMK1 antibody
  • CAMK1 PEN antibody
  • CaMKI alpha antibody
  • MGC120317 antibody
  • MGC120318 antibody
see all

Database links

see all

Anti-CaMKI antibody [EPR2217Y] images:

  Western blot - CaMKI antibody [EPR2217Y] (ab68234)

Western blot - CaMKI antibody [EPR2217Y] (ab68234)

All lanes : Anti-CaMKI antibody [EPR2217Y] (ab68234) at 1/5000 dilution

Lane 1 : SH-SY5Y lysate
Lane 2 : human brain tissue lysate
Lane 3 : mouse brain tissue lysate
Lane 4 : rat brain tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 41 kDa
Observed band size : 41 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CaMKI antibody [EPR2217Y] (ab68234)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CaMKI antibody [EPR2217Y] (ab68234)

ab68234 at 1/100-1/250 dilution staining CaMKI in human kidney tissue sections.

References for Anti-CaMKI antibody [EPR2217Y] (ab68234)

This product has been referenced in:

  • Jiao Xet al. Modulation of neuritogenesis by a protein implicated in X-linked mental retardation. J Neurosci 29:12419-27 (2009).Read more (PubMed: 19812318) »

See 1 publication for this product

Publishing research using ab68234? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"