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Western blot - CaMKI antibody (ab3551)
(enlarge)
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Product Name
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CaMKI antibody
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See all CaMKI antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CaMKI
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Immunogen
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Synthetic peptide: AVEGPRWKQAEDIRDIYD, corresponding to amino acids 1-18 of Human CaMKI.
BLAST 'AVEGPRWKQAEDIRDIYD' with  or 
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Reacts with
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Rat
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Specificity
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Detects CaMKI from rat brain extract.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 41 kDa.
By Western blot, this antibody detects an ~43 kDa protein representing CaMKI from rat brain extract.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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AtT20 cell extract.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
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Relevance
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Calcium / calmodulin-dependent kinases (CaMKs) are a subfamily of the serine/threonine protein kinase family that includes CaMKI, CaMKII, and CaMKIV. There have been identified multiple substrates for CaMKI, including synapsin 1, synapsin 2, CREB, and CFTR, and its activation is dependent upon phosphorylation of Thr 177 by CaMK kinase (CaMKK). Studies suggest that CaMKI plays an important role in retinal function and its expression is regulated by light stimulation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS, 1 mg/ml BSA and 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CaMKI antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CaMKI antibody images, references, products related to ab3551 and other tools.
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CaMKI antibody images:
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 Western blot - CaMKI antibody (ab3551)
CaMKI antibody (ab3551) at 2 µg/ml + Cell lysate prepared from AtT20 cells
Predicted band size : 41 kDa
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Search PubMed (MEDLINE) for references to CaMKI
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CaMKI antibody - more information
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Customer reviews (feedback) regarding CaMKI antibody |
Customer FAQs regarding CaMKI antibody |
Protocols for CaMKI antibody |
Price and availability of products related to CaMKI antibody |
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CaMKI antibody - related products:
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CaMKI antibody - other tools:
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Contact Abcam with a Technical Enquiry about CaMKI antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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