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Anti-CaMKI gamma antibody (ab37972)

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Overview

Product name

Anti-CaMKI gamma antibody
See all CaMKI gamma products (6) ...

Description

Rabbit polyclonal to CaMKI gamma

Tested applications

ELISA, WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow

Immunogen

Synthetic peptide: CSEPTLLKKANKKQNFK conjugated to KLH, corresponding to amino acids 434-450 of Human CAMK1G.

CSEPTLLKKA NKKQNFK

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

NCI-H460 cell lysate, Human hepatocarcinoma tissue.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

The antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

Research areas

Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases

  • Western blot - CAMK1G antibody (ab37972)Western blot - CAMK1G antibody (ab37972) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAMK1G antibody (ab37972)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAMK1G antibody (ab37972) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab37972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/1000

    ELISA: 1/1000

  • ShowHide1 Image

    WB

     WB: 1/100 - 1/500.Detect...Read more →

    WB: 1/100 - 1/500.Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Relevance

CaMKI gamma is a Calcium/calmodulin-dependent protein kinase which belongs to a proposed calcium-triggered signaling cascade. In vitro it phosphorylates transcription factor CREB1. It has been proposed that CaMKI gamma is involved in dendritic outgrowth mediated by CaM-dependant protein kinase kinase.

Cellular localization

Cytoplasm. Golgi apparatus; Golgi membrane; peripheral membrane protein. Cell membrane; peripheral membrane protein.

Alternative names

  • Calcium/calmodulin dependent protein kinase type 1G antibody
  • Calcium/calmodulin-dependent protein kinase type 1G antibody
  • CaM kinase I gamma antibody
  • CaM kinase IG antibody
  • CaMK like CREB kinase III antibody
  • CAMK1G antibody
  • CaMKI gamma antibody
  • CaMKIG antibody
  • CLICK III antibody
see all

Database links

Anti-CaMKI gamma antibody images:

  Western blot - CAMK1G antibody (ab37972)

Western blot - CAMK1G antibody (ab37972)

Anti-CaMKI gamma antibody (ab37972) at 1/100 dilution + NCI-H460 cell lysate

Secondary
HRP-anti-rabbit

Predicted band size : 53 kDa
Observed band size : 53 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAMK1G antibody (ab37972)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAMK1G antibody (ab37972)

ab37972, at a 1/50 dilution, staining CAMK1G in Human hepatocarcinoma tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue. ab37972 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References for Anti-CaMKI gamma antibody (ab37972)

ab37972 has not yet been referenced specifically in any publications.

Publishing research using ab37972? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"