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Immunocytochemistry/ Immunofluorescence - CaMKI (phospho T177) antibody (ab62215)
Immunofluorescence analysis of HeLa cells, using ab62215 (1/500 - 1/1000) staining CaMKI; in the presence and absence of immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CaMKI (phospho T177) antibody(ab62215)
Ab62215 staining human normal occipital cortex. Staining is localised to nuclear and cytoplasmic compartments.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
CaMKI (phospho T177) antibody for Western blot in Mouse (62215)
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