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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Overview
Product name
Anti-CaMKI (phospho T177) antibody
See all CaMKI products (9) ...
Description
Rabbit polyclonal to CaMKI (phospho T177)
Specificity
ab62215 detects CaMKI only when phosphorylated at threonine 177.
Tested applications
IHC-P, ICC/IF, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human CaMKI around the phosphorylation site of threonine 177 (L-S-TP-A-C).
Positive control
HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab62215 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 4 µg/ml
ICC/IF: 1/500 - 1/1000.
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Relevance
Calcium / calmodulin-dependent kinases (CaMKs) are a subfamily of the serine/threonine protein kinase family that includes CaMKI, CaMKII, and CaMKIV. There have been identified multiple substrates for CaMKI, including synapsin 1, synapsin 2, CREB, and CFTR, and its activation is dependent upon phosphorylation of Thr 177 by CaMK kinase (CaMKK). Studies suggest that CaMKI plays an important role in retinal function and its expression is regulated by light stimulation.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- Calcium / calmodulin dependent protein kinase 1 antibody
- Calcium / calmodulin dependent protein kinase I antibody
- Calcium / calmodulin dependent protein kinase type 1 antibody
see all
Database links
- Entrez Gene: 8536 Human
- Entrez Gene: 52163 Mouse
- Entrez Gene: 171503 Rat
- Omim: 604998 Human
- SwissProt: Q14012 Human
- SwissProt: Q91YS8 Mouse
- SwissProt: Q63450 Rat
- Unigene: 434875 Human
see all
Anti-CaMKI (phospho T177) antibody images:
Immunocytochemistry/ Immunofluorescence - CaMKI (phospho T177) antibody (ab62215)
Immunofluorescence analysis of HeLa cells, using ab62215 (1/500 - 1/1000) staining CaMKI; in the presence and absence of immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CaMKI (phospho T177) antibody(ab62215)
Ab62215 staining human normal occipital cortex. Staining is localised to nuclear and cytoplasmic compartments.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-CaMKI (phospho T177) antibody (ab62215)
ab62215 has not yet been referenced specifically in any publications.
Publishing research using ab62215? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"