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Immunocytochemistry/ Immunofluorescence - CaMKI (phospho T177) antibody (ab62215)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CaMKI (phospho T177) antibody(ab62215)
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Product Name
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CaMKI (phospho T177) antibody
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See all CaMKI antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CaMKI (phospho T177)
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Immunogen
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Synthetic phosphopeptide derived from human CaMKI around the phosphorylation site of threonine 177 (L-S-TP-A-C).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab62215 detects CaMKI only when phosphorylated at threonine 177.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
ICC/IF: 1/500 - 1/1000.
IHC-P: Use at a concentration of 4 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
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Relevance
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Calcium / calmodulin-dependent kinases (CaMKs) are a subfamily of the serine/threonine protein kinase family that includes CaMKI, CaMKII, and CaMKIV. There have been identified multiple substrates for CaMKI, including synapsin 1, synapsin 2, CREB, and CFTR, and its activation is dependent upon phosphorylation of Thr 177 by CaMK kinase (CaMKK). Studies suggest that CaMKI plays an important role in retinal function and its expression is regulated by light stimulation.
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CaMKI (phospho T177) antibody (ab62215) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab62215 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against
non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CaMKI (phospho T177) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CaMKI (phospho T177) antibody images, references, products related to ab62215 and other tools.
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CaMKI (phospho T177) antibody images:
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 Immunocytochemistry/ Immunofluorescence - CaMKI (phospho T177) antibody (ab62215)
Immunofluorescence analysis of HeLa cells, using ab62215
(1/500 - 1/1000) staining CaMKI; in the presence and absence of immunizing peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CaMKI (phospho T177) antibody(ab62215)
Ab62215 staining human normal occipital cortex. Staining is localised to nuclear and cytoplasmic compartments.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to CaMKI
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CaMKI (phospho T177) antibody - more information
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