CaMKII alpha 293T Transfected Lysate - (positive control) (ab94251)

Overview

  • Product nameCaMKII alpha 293T Transfected Lysate - (positive control)
  • General notesab94251 is a 293T cell transfected lysate in which Human CaMKII alpha has been transiently over-expressed using a pCMV-CaMKII alpha plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWBmore details

Properties

  • BackgroundFunction: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.

Applications

Our Abpromise guarantee covers the use of ab94251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • CaMKII alpha 293T Transfected Lysate - (positive control) images

    • ab94251 at 15µg/lane on an SDS-PAGE gel
    • All lanes : Anti-CaMKII alpha antibody (ab54925) at 1/500 dilution

      Lane 1 : CaMKII alpha 293T Transfected Lysate - (positive control) (ab94251)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for CaMKII alpha 293T Transfected Lysate - (positive control) (ab94251)

    ab94251 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"