Recombinant
RabMAb

Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880)

Overview

  • Product name
    Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830]
    See all CaMKII alpha + CaMKII beta (phospho T286) primary antibodies
  • Description
    Rabbit monoclonal [EPR1830] to CaMKII alpha + CaMKII beta (phospho T286)
  • Specificity
    Due to the homology between the immunogen peptide and other CAMKII isoforms, ab124880 may cross react with CAMKII delta (KCC2D, 93% homology) and CAMKII gamma (KCC2G, 79% homology)
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CaMKII alpha + CaMKII beta (phospho T286) aa 250-350.

  • Positive control
    • Rat brain lysate (cells untreated/treated with Lambda Phosphatase).
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 54 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 24073199
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Relevance
      Function: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Tissue specificity: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
    • Database links
    • Alternative names
      • Alpha CaMKII antibody
      • Calcium calmodulin dependent protein kinase II antibody
      • Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
      • Calcium/calmodulin dependent protein kinase II antibody
      • Calcium/calmodulin dependent protein kinase II beta antibody
      • Calcium/calmodulin dependent protein kinase IIB antibody
      • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
      • Calcium/calmodulin dependent protein kinase type II beta chain antibody
      • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
      • Calcium/calmodulin-dependent protein kinase (CaM kinase) II antibody
      • Calcium/calmodulin-dependent protein kinase II alpha antibody
      • Calcium/calmodulin-dependent protein kinase II antibody
      • calcium/calmodulin-dependent protein kinase II beta antibody
      • Calcium/calmodulin-dependent protein kinase II-alpha antibody
      • Calcium/calmodulin-dependent protein kinase type II antibody
      • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
      • Calcium/calmodulin-dependent protein kinase type II subunit beta antibody
      • Calcium/calmodulin-dependent protein kinase type IIA antibody
      • CAM 2 antibody
      • CaM kinase II alpha chain antibody
      • CaM kinase II alpha subunit antibody
      • CaM kinase II antibody
      • CaM kinase II beta chain antibody
      • CaM kinase II beta subunit antibody
      • CaM kinase II subunit alpha antibody
      • CaM kinase II subunit beta antibody
      • CaM-kinase II beta chain antibody
      • CAM2 antibody
      • CAMK 2 antibody
      • CaMK II alpha subunit antibody
      • CaMK II beta subunit antibody
      • CaMK-II antibody
      • CaMK-II subunit alpha antibody
      • CaMK-II subunit beta antibody
      • Camk2 antibody
      • Camk2a antibody
      • Camk2b antibody
      • CAMKA antibody
      • CaMKII antibody
      • CaMKIINalpha antibody
      • KCC2_HUMAN antibody
      • KCC2A_HUMAN antibody
      • KCC2B_HUMAN antibody
      see all

    Images

    • All lanes : Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880) at 1/1000 dilution

      Lane 1 : Rat brain lysate untreated
      Lane 2 : Rat brain lysate treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 54 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • La Montanara P  et al. Synaptic synthesis, dephosphorylation, and degradation: a novel paradigm for an activity-dependent neuronal control of CDKL5. J Biol Chem 290:4512-27 (2015). Read more (PubMed: 25555910) »
    • Haolong C  et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). WB ; Human . Read more (PubMed: 24073199) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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