The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 54 kDa).
Use at an assay dependent concentration. PubMed: 24073199
Is unsuitable for ICC,IHC-P or IP.
Function: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Tissue specificity: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
La Montanara P et al. Synaptic synthesis, dephosphorylation, and degradation: a novel paradigm for an activity-dependent neuronal control of CDKL5. J Biol Chem290:4512-27 (2015).
Read more (PubMed: 25555910) »
Haolong C et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One8:e73900 (2013).
Read more (PubMed: 24073199) »