CaMKII alpha antibody [6G9] (ab2725)

Product Name 

CaMKII alpha antibody [6G9]

Product type 

Primary antibodies

Description 

Mouse monoclonal [6G9] to CaMKII alpha

Immunogen 

Partially purified rat CaM kinase II

Reacts with 
(species key)

Ms, Rat

Specificity 

Detects CaM kinase II from rat tissues. This antibody has been shown to detect both the phosphorylated and non-phosphorylated alpha subunit of CaM kinase II only. Does not detect the 60 kDa beta subunit in either phosphorylation state.

Tested applications 
(see key)

ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic; Cell membrane; presynaptic membrane. Cell junction; synapse. Postsynaptic lipid rafts.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK

Relevance 

Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning.

CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1% of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30% of total protein in the post synaptic density (PSD) region. Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

6G9

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.170 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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CaMKII alpha antibody [6G9] images:

References for CaMKII alpha antibody [6G9] (ab2725)

  This product has been used in: (two most recent references)

Leininger E & Belousov AB Recovery of network-driven glutamatergic activity in rat hippocampal neurons during chronic glutamate receptor blockade. Brain Res 1251:87-102 (2009). WB; Mouse. PubMed: 19059386

Jung SC et al. Rapid, bidirectional remodeling of synaptic NMDA receptor subunit composition by A-type K+ channel activity in hippocampal CA1 pyramidal neurons. Neuron 60:657-71 (2008). WB; Mouse. PubMed: 19038222

See all 3 publication references for this product.


If you publish research using ab2725 please let us know so that we can cite the reference on this datasheet.

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