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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Overview
Product name
Anti-CaMKII alpha antibody
See all CaMKII alpha products (11) ...
Description
Rabbit polyclonal to CaMKII alpha
Specificity
ab50202 does not cross-react with CaMKIIß, g and d isoforms (60 kDa).
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Human
Predicted to work with
Cow, Zebrafish
Immunogen
Synthetic peptide: KWQIVHFHRSGAPSVLPH conjugated to KLH, corresponding to C terminal amino acids 461-478 of Rat CaMKII alpha
KWQIVHFHRS GAPSVLPH
Positive control
Synaptosomal fraction of rat brain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000Predicted molecular weight: 54 kDa.(Staining of CaM Kinase IIa in immunoblotting is specifically inhibited with CaM Kinase IIa immunizing peptide. )
IP: Use at an assay dependent dilution. (5 µg using 10 µg of a synaptosomal fraction of rat brain.)
Target
Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localization
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
Target information above from: UniProt accessionQ9UQM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
see all
Database links
- Entrez Gene: 374175 Chicken
- Entrez Gene: 530719 Cow
- Entrez Gene: 815 Human
- Entrez Gene: 12322 Mouse
- Entrez Gene: 25400 Rat
- Entrez Gene: 550436 Zebrafish
- Omim: 114078 Human
- SwissProt: Q08E45 Cow
see all
References for Anti-CaMKII alpha antibody (ab50202)
ab50202 has not yet been referenced specifically in any publications.
Publishing research using ab50202? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"