CaMKII alpha antibody (ab50202)

Product Name 

CaMKII alpha antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to CaMKII alpha

Immunogen 

Synthetic peptide: KWQIVHFHRSGAPSVLPH conjugated to KLH, corresponding to C terminal amino acids 461-478 of Rat CaMKII alpha
BLAST 'KWQIVHFHRSGAPSVLPH' with or

Reacts with 

Hu, Ms, Rat, Chk

Predicted to react 
(species key)

Cow, Zfsh
(Due to sequence homology)

Specificity 

ab50202 does not cross-react with CaMKIIß, g and d isoforms (60 kDa).

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IP: 5 µg using 10 µg of a synaptosomal fraction of rat brain.
WB: 1/5000 using synaptosomal fraction of rat brain. Predicted molecular weight: 54 kDa. Staining of CaM Kinase IIa in immunoblotting is specifically inhibited with CaM Kinase IIa immunizing peptide.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Synaptosomal fraction of rat brain

Cellular localization 

Cytoplasmic; Cell membrane; presynaptic membrane. Cell junction; synapse. Postsynaptic lipid rafts.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK

Relevance 

Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Form 

Liquid

Concentration 

Batch dependent within range: 5.50 - 6.20 mg/ml

Concentration of lot no.     is  

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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